▎ 摘　　要

NOVELTY - A dual mode of fluorescence and colorimetric based alkaline phosphatase activity detecting method involves preparing a graphene quantum dot, placing alkaline phosphatase solution into excess L-ascorbic acid-2-phosphate solution, and placing mixture in an alkaline buffer solution to co-incubate to form L-ascorbate. Double distilled water, graphene quantum dot and silver nitrate solution are mixed into the mixture, silver nanoparticles are deposited on rare quantum dots of graphite, followed by detecting alkaline phosphatase activity according to intensity. USE - Method for detecting dual mode of fluorescence and colorimetric based alkaline phosphatase activity. ADVANTAGE - The method enables reducing the error caused by environmental fluctuations and ensuring the reliability of the measurement results. DETAILED DESCRIPTION - A dual mode of fluorescence and colorimetric based alkaline phosphatase activity detecting method involves preparing a graphene quantum dot, placing alkaline phosphatase solution into an excess L-ascorbic acid-2-phosphate solution, and placing mixture in an alkaline buffer solution to co-incubate to form L-ascorbate. Double distilled water, graphene quantum dot and silver nitrate solution are mixed into the mixture, silver nanoparticles are deposited on the surface of the rare quantum dots of graphite, and the fluorescence intensity of the surface of the graphite dots is gradually decreased, and the absorbance of the silver nanoparticles deposited on the surface of the graphene quantum dots gradually increase followed by detecting alkaline phosphatase activity according to the fluorescence intensity or absorbance Changes. An INDEPENDENT CLAIM is also included for a sensor for detecting alkaline phosphatase activity.