▎ 摘　　要

NOVELTY - Preparing glucose dehydrogenase involves dissolving glucose dehydrogenase in a buffer solution containing potassium bromide, and pouring the buffer solution into a dialysis bag for dialysis, dialyzing again in phosphate buffer solution to obtain deprostheticized glucose dehydrogenase and chemically modifying the deprosthetized glucose dehydrogenase to obtain the chemically modified glucose dehydrogenase. A mixed reaction of graphene oxide is added with carboxyl group, N6-(6-aminohexyl)flavin adenine dinucleotide, carbodiimide and N-hydroxysuccinimide. The reaction solution is centrifuged, and after the centrifugation is completed, cleaning and purification are performed to obtain graphene oxide with N6-(6-aminohexyl)flavin adenine dinucleotide. USE - Method for preparing glucose dehydrogenase for preparing glucose sensor (claimed). ADVANTAGE - The method enables to prepare glucose dehydrogenase, where direct electrochemistry of glucose dehydrogenase is realized, which fundamentally eliminates the restriction of oxygen on glucose detection and improves the accuracy of glucose dynamic detection. DETAILED DESCRIPTION - Preparing glucose dehydrogenase involves dissolving glucose dehydrogenase in a buffer solution containing potassium bromide, and pouring the buffer solution into a dialysis bag for dialysis, dialyzing again in phosphate buffer solution to obtain deprostheticized glucose dehydrogenase and chemically modifying the deprosthetized glucose dehydrogenase to obtain the chemically modified glucose dehydrogenase. A mixed reaction of graphene oxide is added with carboxyl group, N6-(6-aminohexyl)flavin adenine dinucleotide, carbodiimide and N-hydroxysuccinimide. The reaction solution is centrifuged, and after the centrifugation is completed, cleaning and purification are performed to obtain graphene oxide with N6-(6-aminohexyl)flavin adenine dinucleotide. A mixed reaction of the chemically modified glucose dehydrogenase, the graphene oxide is performed with N6-(6-aminohexyl)flavin adenine dinucleotide and calcium chloride. After the reaction finishes, sodium borohydride is added and reacted. After the reaction, centrifugation is performed, and the obtained glucose dehydrogenase is washed and separated to obtain a reconstituted glucose dehydrogenase. An INDEPENDENT CLAIM is included for a method for preparing glucose sensor, which involves mixing glucose dehydrogenase with the chemical cross-linking agent for 40 to 100 minutes, coating chemically cross-linked glucose dehydrogenase on the surface of the electrode to obtain a glucose sensing membrane, coating the biocompatible membrane solution on the glucose sensing membrane and drying.