▎ 摘　　要

NOVELTY - Detecting multi-drug resistant microorganism comprises (i) converting protein signal by taking the nucleic acid aptamer, adding blocker solution X, and reacting and cooling, adding mixed product A into the magnetic bead solution, mixing with buffer, and incubating to obtain aptamer-functionalized magnetic beads, and adding bacterial solution to be tested into aptamer functionalized magnetic beads for displacement reaction, discarding the magnetic beads and taking the supernatant to obtain blocker solution Y; (ii) mixing amplification of nucleic acid signal by restriction endonuclease, DNA polymerase, buffer G, molecular enhancer, amplification template, dNTP, buffer H and blocker solution Y, and performing exponential amplification reaction to obtain amplification reaction product C; (iii) detecting nucleic acid signal by adding fluorescent probe and amplification reaction product C into the transition metal dihalogen compound nanosheet solution, and measuring fluorescence intensity. USE - The method is useful for detecting multidrug resistant microorganism. ADVANTAGE - The method inhibits the non-specific amplification reaction, reduces the intensity of the background signal, is simple and efficient to operate, can achieve the purpose of background-free, fast, and ultra-sensitive detection, and can solve the problem of long detection cycle, low sensitivity, background signal in the detection result, and inaccurate interpretation of the result caused by the influence of subjective factors in the traditional detection technology, and isothermal amplification reaction has serious non-specific amplification and other key technical problems. DETAILED DESCRIPTION - Detecting multi-drug resistant microorganism comprises (i) converting protein signal by taking the nucleic acid aptamer, adding blocker solution X, and reacting in water bath and cooling to obtain the mixed product A, adding mixed product A into the magnetic bead solution, mixing with the buffer, and incubating to obtain the aptamer-functionalized magnetic beads, and adding the bacterial solution to be tested into the aptamer functionalized magnetic beads for displacement reaction, discarding the magnetic beads and taking the supernatant to obtain blocker solution Y; (ii) mixing amplification of nucleic acid signal by restriction endonuclease, DNA polymerase, buffer G, molecular enhancer, amplification template, dNTP, buffer H and blocker solution Y, and performing exponential amplification reaction to obtain amplification Reaction product C; (iii) detecting nucleic acid signal by adding fluorescent probe and amplification reaction product C into the transition metal dihalogen compound nanosheet solution or the graphene nanosheet solution, and measuring fluorescence intensity in a water bath.