• 专利标题:   Kit for detecting feline pancreas-specific lipase by homogeneous chemiluminescence method, comprises detection solution, which includes DNA1-fPL antibody 1 conjugate, DNA2-fPL antibody 2 conjugate, DNA3 labeled acridinium ester AE and graphene oxide GO combined with antioxidant AOD.
  • 专利号:   CN115343466-A
  • 发明人:   ZHAO B, CHENG S, CAO D
  • 专利权人:   NANJING QIUPU BIOTECHNOLOGY CO LTD
  • 国际专利分类:   G01N021/76, G01N033/537, G01N033/541
  • 专利详细信息:   CN115343466-A 15 Nov 2022 G01N-033/537 202307 Chinese
  • 申请详细信息:   CN115343466-A CN10978424 16 Aug 2022
  • 优先权号:   CN10978424

▎ 摘  要

NOVELTY - Kit comprises detection solution, which includes DNA1-fPL antibody 1 conjugate, DNA2-fPL antibody 2 conjugate, DNA3 labeled acridinium ester AE and graphene oxide GO combined with antioxidant AOD. The DNA1 contains 51 bases, 3' is modified with NH2C6, and the coupling agent sulfosuccinic acid diisooctanoic acid is covalently bonded to the amino group on the fPL antibody 1 to form a DNA1-fPL antibody 1 conjugate. The DNA2 contains 50 bases, the 5' end is modified with NH2C5, and the amino group on the fPL antibody 2 is covalently bonded with sulfosuccinic acid diisooctanoic acid to form a DNA2-fPL antibody 2 conjugate. The DNA3 contains 18 bases, and the 5' end is modified with acridinium ester AE. 7 bases complementary between DNA1 and DNA2, 6 bases complementary between DNA1 and DNA3, and 6 bases complementary between DNA2 and DNA3 are provided. USE - Kit for detecting feline pancreas-specific lipase by homogeneous chemiluminescence method. ADVANTAGE - The kit has simple detection, high detection accuracy and sensitivity, lesser types of protein protection components, and high solution stability.