• 专利标题:   Reagent used for rapid detection of heparin comprises chromone Schiff base/mitoxantrone complex or chromone Schiff base/adriamycin complex, where chromone Schiff base/mitoxantrone complex or chromone Schiff base/adriamycin complex is prepared by using graphene quantum dots aqueous solution.
  • 专利号:   CN115290609-A
  • 发明人:   FANG Y, LIU B, ZHOU C, TANG S, QIAN L, WANG Y, WANG Z, CHENG R
  • 专利权人:   UNIV WENZHOU MEDICAL
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN115290609-A 04 Nov 2022 G01N-021/64 202203 Chinese
  • 申请详细信息:   CN115290609-A CN11579764 22 Dec 2021
  • 优先权号:   CN11579764

▎ 摘  要

NOVELTY - Reagent comprises chromone Schiff base/mitoxantrone complex or chromone Schiff base/adriamycin complex solution, where the chromone Schiff base/mitoxantrone complex or chromone Schiff base/adriamycin complex is prepared by placing graphene quantum dots aqueous solution with a concentration of 0.1-20 mg/mL in a container, stirring the solution at a constant speed, adding a certain amount of catalyst, dripping alcohol solution of the chromone compound, heating at 45-80℃ for 2-24 hours and using a dialysis bag with a molecular weight of 1000 Da in alcohol water mixed solution for three days to obtain a chromone Schiff base, mixing the chromone Schiff base with mitoxantrone or adriamycin to obtain the chromone Schiff base/mitoxantrone complex chromone Schiff base/adriamycin complex. USE - The reagent is used for rapid detection of heparin. ADVANTAGE - The reagent adopts the chromone Schiff base/mitoxantrone or adriamycin complex which is simple to synthesize and has selectivity to heparin. The reagent is convenient, has high sensitivity, can quickly detect heparin in normal temperature aqueous solution environment, adopts the method which is simple in process and convenient in operation, and has strong practicability and broad application prospects. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for fast detecting heparin comprising taking the reagent, adding 10-7 M to 10-3 M of the substance to be tested and using fluorescence photometry to test, where the fluorescence spectrum of test solution at EM409 nm.