▎ 摘 要
NOVELTY - Reagent comprises chromone Schiff base/mitoxantrone complex or chromone Schiff base/adriamycin complex solution, where the chromone Schiff base/mitoxantrone complex or chromone Schiff base/adriamycin complex is prepared by placing graphene quantum dots aqueous solution with a concentration of 0.1-20 mg/mL in a container, stirring the solution at a constant speed, adding a certain amount of catalyst, dripping alcohol solution of the chromone compound, heating at 45-80℃ for 2-24 hours and using a dialysis bag with a molecular weight of 1000 Da in alcohol water mixed solution for three days to obtain a chromone Schiff base, mixing the chromone Schiff base with mitoxantrone or adriamycin to obtain the chromone Schiff base/mitoxantrone complex chromone Schiff base/adriamycin complex. USE - The reagent is used for rapid detection of heparin. ADVANTAGE - The reagent adopts the chromone Schiff base/mitoxantrone or adriamycin complex which is simple to synthesize and has selectivity to heparin. The reagent is convenient, has high sensitivity, can quickly detect heparin in normal temperature aqueous solution environment, adopts the method which is simple in process and convenient in operation, and has strong practicability and broad application prospects. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for fast detecting heparin comprising taking the reagent, adding 10-7 M to 10-3 M of the substance to be tested and using fluorescence photometry to test, where the fluorescence spectrum of test solution at EM409 nm.