▎ 摘　　要

NOVELTY - Synthetically fluorescent nanoprobes based on glutathione comprises mixing small molecular polycyclic aromatic hydrocarbons with concentrated nitric acid for nitration reaction at room temperature for 12 hours, adding ultrapure water, diluting, and filtering to obtain orange product; dispersing the orange product in an alkaline solution, ultrasonically mixing, and transferring mixture to a reactor for hydrothermal reaction for 10 hours, cooling to room temperature naturally, filtering and dialyzing filtrate to obtain graphene quantum dots rich in hydroxyl groups on the surface, which were denoted as graphene quantum dots-OH; and dispersing the obtained graphene quantum dots-carboxylic acids, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the dispersion, adjusting pH, stirring at room temperature for 30 minutes, adding N-hydroxysuccinimide, stirring uniformly, adding GSH, adjusting the pH, stirring the reaction in dark, and dialyzing. USE - The method is useful for synthetically fluorescent nanoprobes based on glutathione in tracing anti-oxidative stress function active microorganism BosethiooxidansBI-42, and useful as fluorescent substrate for drug delivery, biomedical imaging, ultra-sensitive biosensor, and microorganism detection. ADVANTAGE - The fluorescent nano-probe structure shows excellent fluorescent characteristics and specificity. DETAILED DESCRIPTION - Synthetically fluorescent nanoprobes based on glutathione comprises mixing small molecular polycyclic aromatic hydrocarbons with concentrated nitric acid for nitration reaction at room temperature for 12 hours, adding ultrapure water, diluting, filtering acid with a microporous membrane to obtain orange product; dispersing the orange product in an alkaline solution, ultrasonically mixing, and transferring the mixture to a reactor for hydrothermal reaction for 10 hours, cooling to room temperature naturally, filtering with a microporous membrane, and dialyzing the filtrate to obtain graphene quantum dots rich in hydroxyl groups on the surface, which were denoted as graphene quantum dots-OH; dispersing the obtained graphene quantum dots-OH, adding sodium hydroxide and ClCH2COONa to the dispersion liquid, where the mass ratio of graphene quantum dots-OH, sodium hydroxide and ClCH2COONa is 0.015:1.2:1.0, sonicating for 3 hours, and adjusting pH of the reaction solution to neutral using dilute hydrochloric acid and dialyzing reaction solution to obtain graphene quantum dots with rich carboxyl groups on surface, denoted as GQDs-COOH; and dispersing the obtained graphene quantum dots-carboxylic acids, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to the dispersion, adjusting the pH to 5, and stirring at room temperature for 30 minutes, adding N-hydroxysuccinimide, stirring uniformly, adding GSH, adjusting the pH to 9, and stirring the reaction in the dark for 48 hours, and dialyzing to obtain nanoparticle-GSH fluorescent probe. DESCRIPTION OF DRAWING(S) - The drawing shows the present invention graphene quantum dot GQDs-COOH and GSH-GQDs of the synthetic process sketch.