▎ 摘　　要

NOVELTY - Method for preparing immunosensor for detecting hepatitis B virus markers, involves preparing a porous graphene-loaded gold nanoparticle modified electrode and a molybdenum disulfide (MoS2).cuprous oxide (Cu2O)/platinum(Pt)-Ab2 detection antibody incubation solution in which the hepatitis B virus marker antibody (Ab1) is immobilized. USE - The method is useful for preparing immunosensor for detecting hepatitis B virus marker chosen from HBe, HBs and HBc (all claimed). ADVANTAGE - The method prepares immunosensor which detects hepatitis B virus marker with strong specificity, high sensitivity and low detection limit. DETAILED DESCRIPTION - Method for preparing immunosensor for detecting hepatitis B virus markers, involves preparing a porous graphene-loaded gold nanoparticle modified electrode and a molybdenum disulfide (MoS2).cuprous oxide (Cu2O)/platinum(Pt)-Ab2 detection antibody incubation solution in which the hepatitis B virus marker antibody (Ab1) is immobilized, where the porous graphene-loaded gold nanoparticle-modified electrode in which the hepatitis B virus marker antibody Ab1 is immobilized is prepared by polishing glass carbon electrode with diameter of 3-5 mm with aluminum oxide polishing powder, cleaning using ultra-pure water, adding 6 mu l 0.5-2 mg/ml porous graphene-loaded gold nanoparticles solution on the electrode surface, drying, rinsing with ultra-pure water, drying, continuously dripping adding 6 mu l 8-12 mu g/ml hepatitis B virus marker antibody Ab1 solution on the electrode surface, maintaining in a refrigerator at 4 degrees C, rinsing using ultra-pure water to remove unbound antibody, adding 3-5 mu l 0.1-1 %mass bovine serum albumin (BSA) solution to the electrode surface on the non-specific active sites, and drying by maintaining in the refrigerator at 4 degrees C. An INDEPENDENT CLAIM is included for use of immunosensor for detecting hepatitis B virus markers, by using electrochemical workstation to test three-electrode system, saturated calomel electrode for reference electrode, platinum wire electrode for auxiliary electrode, using the prepared immunosensor as working electrode, testing with 10 ml 50 mmol/l pH 5.1-8.6 phosphate buffer solution, using time-dependent current method to detect the hepatitis B virus markers with input voltage of -0.4 V, sampling interval of 0.1 second, and running time of 300 second, stabilizing, and injecting 10 mu l 5 mol/l hydrogen peroxide solution every 10 seconds into 10 ml 50 mmol/l pH 6.98 phosphate buffer solution to record current change.