▎ 摘　　要

NOVELTY - Optical PCR system for production of a conductive polymer-based optical PCR plate using a roll-to-roll device, comprises a roll-to-roll device for production of a molecular-based photonic PCR plate comprising a conductive polymer having several reaction spaces in which a nucleic acid amplification reaction occurs by light-to-heat conversion by surface plasmon phenomenon of conductive polymer according to light irradiation, a light source for irradiating light to induce a nucleic acid amplification reaction to conductive polymer-based optical PCR plate produced in the roll-to-roll device, and a photodetector for detecting nucleic acid amplification reaction in the reaction space in real time. USE - The optical PCR system is useful for producing conductive polymer-based optical PCR plate, which is used for performing real-time detection of target nucleic acids (claimed) such as DNA and RNA. ADVANTAGE - The optical PCR system can mass-produces conductive polymer-based optical PCR plate at low cost within a short period of time through roll-to-roll process. DETAILED DESCRIPTION - Optical PCR system for production of a conductive polymer-based optical PCR plate using a roll-to-roll device, comprises a roll-to-roll device for production of a molecular-based photonic PCR plate (10) comprising a conductive polymer having several reaction spaces (S) in which a nucleic acid amplification reaction occurs by light-to-heat conversion by surface plasmon phenomenon of conductive polymer according to light irradiation, a light source (20) for irradiating light to induce a nucleic acid amplification reaction to conductive polymer-based optical PCR plate produced in the roll-to-roll device, and a photodetector (30) for detecting nucleic acid amplification reaction in the reaction space in real time. The conductive polymer-based optical PCR plate is transferred from the roll-to-roll device toward the light source, and light is irradiated from the light source to the reaction space during the transport process. The roll-to-roll device comprises an imprinting unit (11) for imprinting a reaction space having a plasmonic thin film for plasmonic photothermal light-to-heat conversion of a conductive polymer on a substrate in an nasteriskm array in form of a microwell, and a laminating unit (12) for laminating an upper portion of the reaction space with a film. An INDEPENDENT CLAIM is included for method for real-time detection of a target nucleic acid using the prepared optical PCR plate, which involves (a) producing a conductive polymer-based optical PCR plate having several reaction spaces in which a nucleic acid amplification reaction occurs by light-to-thermal conversion by a surface plasmon phenomenon of a conductive polymer according to light irradiation using a roll-to-roll device, (b) transferring the prepared conductive polymer-based optical PCR plate to a light source and irradiating light into the reaction space, and (c) detecting the nucleic acid amplification reaction in the reaction space in real time with a photodetector, and producing conductive polymer-based optical PCR plate using a roll-to-roll device, where the step (a) involves imprinting a reaction space comprising a plasmonic conductive polymer thin film for plasmonic photothermal light-to-thermal conversion on a substrate in an nasteriskm array in the form of a fine well, injecting a biological sample and a reagent for amplification of a target nucleic acid in the biological sample into the reaction space, and laminating upper portion of the reaction space with a film while producing a conductive polymer-based optical PCR plate with a roll-to-roll device. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of optical PCR system. PCR plate (10) Imprinting unit (11) Laminating unit (12) Light source (20) Photodetector (30) Reaction space (S)