▎ 摘　　要

NOVELTY - Method for preparing mantis shrimp eyeball paraffin section involves (a) collecting materials by removing eye stalk with higher hardness, reserving cornea part with lower hardness but closest to the cone, using clean ophthalmic scissors to cut off the eyeballs along the base of the eye stalks of mantis shrimp and soaking them in filtered seawater, using corneal scissors and special forceps for ophthalmology to dissect and remove the shell of the eye stalk, and keeping the shell of the cornea, (b) fixing with paraformaldehyde, (c) performing dehydration treatment, (d) embedding the tissue block soaked in wax with modified paraffin, (e) using a microtome to slice the sample continuously, (f) flattening the slices in a 37°C water bath, (g) dewaxing and rehydrating after incubating at 37°C for 6-12 hours, and (h) staining the sample, and using 50% glycerin sterile aqueous solution to seal the sample for preservation. USE - The method is useful for preparing mantis shrimp eyeball paraffin section. ADVANTAGE - The method enables to prepare shrimp cricket eyeball paraffin slice, which is easy to dissection caused by dehydration of wax is not complete, the shell is hard damage blade, slice generating fragment and other technical bottleneck. DETAILED DESCRIPTION - Method for preparing mantis shrimp eyeball paraffin section involves (a) collecting materials by removing eye stalk with higher hardness, reserving cornea part with lower hardness but closest to the cone, using clean ophthalmic scissors to cut off the eyeballs along the base of the eye stalks of mantis shrimp and soaking them in filtered seawater, using corneal scissors and special forceps for ophthalmology to dissect and remove the shell of the eye stalk, and keeping the shell of the cornea, (b) fixing with 4-6% paraformaldehyde at room temperature for 25-35 minutes, (c) performing dehydration treatment, (d) embedding the tissue block soaked in wax with modified paraffin, (e) using a microtome to slice the sample continuously, with a slice thickness of 4 μm, (f) flattening the slices in a 37°C water bath to spread them on the poly-lysine-treated glass slides, (g) dewaxing and rehydrating after incubating at 37°C for 6-12 hours, and (h) staining the sample, and using 50% glycerin sterile aqueous solution to seal the sample for preservation.