▎ 摘　　要

NOVELTY - Preparation of three-dimensional metal-enhanced fluorescence lead (II) ion biosensor by adding three-dimensional metal-enhanced fluorescent paper chip hydrophobic wax printing area and hydrophilic area into wax printer, printing hydrophobic pattern, and heating to obtain hydrophobic layer; growing decahedral silver in the hydrophilic area, fixing with catalytic chain functionalized graphene quantum dots, washing, dropping mixed dye and cerium dioxide functionalized lead (II) ion, and washing; and adding lead (II) ion, and determining fluorescence intensity of graphene quantum dots and dyes. USE - Method for preparation of three-dimensional metal-enhanced fluorescence lead (II) ion biosensor (claimed). ADVANTAGE - The method achieves portable, reliable and highly-sensitive detection of lead (II) ion. DETAILED DESCRIPTION - Preparation of three-dimensional metal-enhanced fluorescence lead (II) ion biosensor comprises: (A) selecting three-dimensional metal-enhanced fluorescent paper chip hydrophobic wax printing area and hydrophilic area, putting into wax printer, printing hydrophobic pattern, and heating to 60-150 degrees C until wax melts and soaks the entire paper to obtain hydrophobic layer; (B) growing decahedral silver in the hydrophilic area, fixing with catalytic chain functionalized graphene quantum dots as DNA1, washing with buffer solution, dropping mixed dye and cerium dioxide functionalized lead (II) ion as 17DS, and washing with buffer solution; and (C) adding different concentrations of lead (II) ion, putting into fluorescent device, determining fluorescence intensity of graphene quantum dots and dyes under different concentrations of lead (II) ion, and drawing relationship between the change of the ratio of the two fluorescence intensities and concentration of lead (II) ion.