• 专利标题:   Method for chiral separation of amino acids on pectinase-modified capillary silica monolithic column by functionalizing graphene oxide-modified silica gel monolithic column using coupling agent, injecting pectinase solution to obtain pectinase-modified capillary silica monolithic column and applying.
  • 专利号:   CN116024279-A
  • 发明人:   GUO J, ZHU X, YANG G, HONG T, LIU X, CAI Z
  • 专利权人:   UNIV CHANGZHOU
  • 国际专利分类:   B01D015/22, B01J020/29, C12P013/10, C12P013/22, C12P013/24, C12P041/00
  • 专利详细信息:   CN116024279-A 28 Apr 2023 C12P-013/22 202346 Chinese
  • 申请详细信息:   CN116024279-A CN11308002 25 Oct 2022
  • 优先权号:   CN11308002

▎ 摘  要

NOVELTY - A method for chiral separation of amino acids on a pectinase-modified capillary silica monolithic column comprises (S1) preparing a graphene oxide-modified silica gel monolithic column, functionalizing it using a coupling agent, and injecting a pectinase solution into the functionalized monolithic column for reacting to obtain a pectinase-modified capillary silica monolithic column, and (S2) applying the pectinase-modified capillary silica monolithic column obtained in step (S1) to the separation of amino acid enantiomers, where the amino acid is one of histidine, tryptophan or arginine. USE - The method is used for chiral separation of amino acids on a pectinase-modified capillary silica monolithic column. ADVANTAGE - The method is simple to operate, and establishes a new and highly selective separation and detection technology of amino acid enantiomers.