• 专利标题:   Blood vessel-mimicking microfluidic chip for cell co-culture, and for analyzing photothermal therapeutic effect on cancer cells, comprises cell culture channels and cell co-culture channel as cell culture sections, and bridge channels.
  • 专利号:   US2021032584-A1, KR2021014464-A
  • 发明人:   CHUNG B G, MUN S G, LEE J M, CHOI H W, LIM J H, KIM E J, MUN S, LEE J
  • 专利权人:   UNIV SOGANG RES FOUND, UNIV SOGANG RES BUSINESS DEV FOUND
  • 国际专利分类:   B01L003/00, C12M003/06, C12N005/071, G01N033/50, G01N033/543, A61K041/00, C12M001/00, C12N005/09
  • 专利详细信息:   US2021032584-A1 04 Feb 2021 C12M-003/06 202133 English
  • 申请详细信息:   US2021032584-A1 US666577 29 Oct 2019
  • 优先权号:   KR092581

▎ 摘  要

NOVELTY - Blood vessel-mimicking microfluidic chip for cell co-culture, comprises: (a) a 1st cell culture channel, a 2nd cell culture channel, and a cell co-culture channel, as cell culture sections; and (b) bridge channels connected to the cell culture channels. The cell co-culture channel is disposed between the 1st cell culture channel and the 2nd cell culture channel and the 1st cell culture channel, the 2nd culture channel, and the cell co-culture channel are connected through hollow tubular bridge channels. USE - The blood vessel-mimicking microfluidic chip is useful for cell co-culture; and for analyzing a photothermal therapeutic effect on cancer cells (all claimed). It can also be used in studies on cancer metastasis, intravenous injection environments for cancer treatment, and photothermal therapeutic effects on cancer cell. ADVANTAGE - In the method for analyzing a photothermal therapeutic effect on cancer cells by using the blood vessel-mimicking microfluidic chip for cell co-culture, the overlapping contents are omitted to avoid excessive complexity of the specification due to repetitive descriptions. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for analyzing a photothermal therapeutic effect on cancer cells, which involves: (a) preparing a blood vessel-mimicking microfluidic chip for cell co-culture, the microfluidic chip comprising: (i) a 1st cell culture channel, a 2nd cell culture channel, and a cell co-culture channel, as cell culture sections; and (ii) bridge channels connected to the cell culture channels, where the cell co-culture channel is disposed between the 1st cell culture channel and the 2nd cell culture channel and the 1st cell culture channel, the 2nd culture channel, and the cell co-culture channel are connected through hollow tubular bridge channels; (b) injecting vascular endothelial cells and cancer cells into the 1st cell culture channel and the 2nd cell culture channel, respectively, and injecting vascular endothelial cells and cancer cells into the cell co-culture channel, followed by culture; (c) injecting nanoparticles showing a photothermal effect into the 1st cell culture channel, the 2nd cell culture channel, or the cell co-culture channel, followed by culture; and (d) subjecting the microfluidic chip to laser irradiation to analyze the degrees of survival and death of the cancer cells.