▎ 摘　　要

NOVELTY - Single cell function assessment kit comprises a micro-cavity array chip, and an antibody barcode chip printed with a functional capture antibody, a functional detection antibody, a bio-affinity modification reagent, a fluorescent conjugate, an antibody buffer, an antibody blocking solution and a cell buffer, is new. USE - Used as single cell function assessment kit. ADVANTAGE - The kit can realize rapid, accurate, high-throughput, and relatively economical sterile airtight micro-cavity. The method has low detection cost, accurate results, high detection sensitivity, and good application prospects. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for method for evaluating single cell function comprising (1) using trypsin to digest the cell tissue first for adherent cells, adding fetal bovine serum, terminating the digestion, shaking, mixing, centrifuging for 2-8 minutes, re-suspending with cell detergent until pale white cell pellet appears at the bottom of the tube, discarding the supernatant, re-suspending the pellet with cell detergent, centrifuging for 2-6 minutes for suspended cells, re-suspending with cell detergent until a pale white cell pellet appears at the bottom of the tube, discarding the supernatant, and re-suspending the pellet with cell detergent, (2) using the graphene nano-material coupling agent to bind to the hydroxy group after plasma hydroxylated the micro-cavity array chip to realize the aminated micro-cavity array chip, using graphene nano-material modifier to modify the micro-cavity array chip with graphene nano-material, using the biological modifier to modify the micro-cavity array chip with biological affinity, and finally using the cell buffer to infiltrate and drying, (3) soaking in antibody blocking solution for 10-20 minutes, centrifuging and drying, (4) uniformly printing cells in the micro-cavity of the preprocessed microcavity array chip, (5) covering the antibody barcode chip on the micro-cavity array chip to form a closed chamber, adding in a carbon dioxide incubator with a volume concentration of 5% at 37 degrees C, and waiting for the cells to attach and secrete proteins for 8-16 hours, (6) removing the antibody barcode chip, washing with antibody buffer, spreading mixed functional detection antibodies on the antibody barcode chip, incubating for 10-30 minutes; then rinse with antibody buffer, taking a certain amount of fluorescent conjugate and spread it on the antibody barcode chip, and incubating in the dark for 20-40 minutes before detecting, and (7) covering the fluorescence value of various secreted signals into various secreted protein amounts through the protein quantitative formula.