• 专利标题:   Measuring molecular oxygen comprises contacting a test sample with luciferase composition comprising lipid-functionalized graphene or lipid-functionalized fullerene and luciferase.
  • 专利号:   US2015044710-A1
  • 发明人:   DASGUPTA A K, RAY S, CHATTERJEE A, BHATTACHARYYA T, BHADURI S, BOSE A
  • 专利权人:   UNIV CALCUTTA
  • 国际专利分类:   A61B005/1455, C12Q001/66
  • 专利详细信息:   US2015044710-A1 12 Feb 2015 C12Q-001/66 201513 Pages: 23 English
  • 申请详细信息:   US2015044710-A1 US455387 08 Aug 2014
  • 优先权号:   INKO00942

▎ 摘  要

NOVELTY - Measuring molecular oxygen comprises contacting a first sample with at least one luciferase composition, where the luciferase composition comprises at least one lipid-functionalized graphene or lipid-functionalized fullerene and at least one luciferase, where the luciferase is entrapped within the lipid-functionalized graphene or the lipid-functionalized fullerene, where the entrapped luciferase is not linked to the lipid-functionalized graphene or the lipid-functionalized fullerene; and detecting luminescence. USE - The method and biosensor device are used for measuring oxygen (claimed). ADVANTAGE - The method for measuring oxygen does not require elaborate chemical or electrochemical techniques. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is a biosensor device for measuring oxygen comprising: at least two measuring chambers comprising tops, where the measuring chamber contains a mixture of compounds, where the mixture of compounds comprises at least one lipid-functionalized graphene or lipid-functionalized fullerene and at least one luciferase, where the luciferase is entrapped within the lipid-functionalized graphene or the lipid-functionalized fullerene, where the entrapped luciferase is not linked to the lipid-functionalized graphene or the lipid-functionalized fullerene; at least two removable vessels for receiving a sample, where the vessels have a removable top and a needle positioned on a bottom, where the vessel and the measuring chamber can be connected by inserting the needle into the top of the measuring chamber; a housing; a sample holder positioned within the housing, where the measuring chamber can be inserted into the sample holder; a transparent lens positioned on the sample holder; a photon detector positioned within the housing; a signal processor, positioned within the housing, where the photon detector and signal processor are connected; and a display unit connected to the signal processor.