• 专利标题:   Nafion/glucose oxidase/graphene oxide/nano porous platinum electrode for use in rapid and sensitive detection of glucose in plant fruit, prepared by taking platinum wire electrode as working electrode, and soaking in graphene oxide solution.
  • 专利号:   CN112485312-A
  • 发明人:   LONG Y, WU B, XU H, YANG L, LI Y, ZHOU H, SHI Y, YAO Z, DENG H
  • 专利权人:   UNIV SOUTH CHINA AGRIC
  • 国际专利分类:   G01N027/327, C25D007/00, C25D015/00, C25F003/02
  • 专利详细信息:   CN112485312-A 12 Mar 2021 G01N-027/327 202128 Pages: 16 Chinese
  • 申请详细信息:   CN112485312-A CN11132569 21 Oct 2020
  • 优先权号:   CN11132569

▎ 摘  要

NOVELTY - Nafion/glucose oxidase/graphene oxide/nano porous platinum electrode is prepared by electrochemical corrosion of nafion/glucose oxidase/graphene oxide/nano porous platinum electrode, forming surface of platinum wire with nano porous structure, obtaining nano porous platinum electrode, taking obtained nano-porous platinum electrode as working electrode, platinum wire electrode as counter electrode, silver/silver chloride electrode as reference electrode, soaking into ultrasonic treatment of graphene oxide aqueous solution, using cyclic voltammetry to deposit graphene oxide on surface of nano porous platinum electrode, preparing graphene oxide/nano porous platinum electrode, soaking obtained graphene oxide/nano-porous platinum electrode in phosphate-buffered saline solution of glucose oxidase at 0-4 degrees C, fixing glucose oxidase on electrode to obtain glucose oxidase/graphene oxide/nano porous platinum electrode, coating and drying. USE - The method is useful in detecting glucose in plant body. ADVANTAGE - The electrode has convenient operation, low cost, low detection limit, high test sensitivity, accuracy, reproducibility, good repeatability and good stability.