▎ 摘　　要

NOVELTY - Preparation of hemoglobin modified alpha -zirconium phosphate (ZrP)-graphene foam reference electrode comprises mixing potassium nitrate and graphite powder in concentrated sulfuric acid, adding potassium permanganate, filtering solution, washing with dilute hydrochloric acid, centrifuging solution with deionized water, drying to obtain graphene oxide, placing graphene oxide in deionized water, performing ultrasonic treatment, adding hydrazine hydrate, soaking in deionized water, freeze-drying to obtain graphene foam, adding zirconium (IV) oxychloride octahydrate and graphene foam to deionized water, filtering solution, washing with anhydrous alcohol and ionic water, drying sample to obtain alpha -ZrP-graphene foam, adding alpha -ZrP/graphene foam material in deionized water, polishing glassy carbon electrode with aluminum oxide powder, coating suspension droplet on working electrode with liquid transfer gun, modifying hemoglobin solution on working electrode and drying. USE - The preparation of hemoglobin modified ZrP-graphene foam reference electrode is used for electrochemical hydrogen peroxide sensor composed of reference electrode (claimed). ADVANTAGE - The sensor has good sensing performance and is not easy to be affected. DETAILED DESCRIPTION - Preparation of hemoglobin modified alpha -zirconium phosphate (ZrP)-graphene foam reference electrode comprises mixing potassium nitrate and graphite powder in concentrated sulfuric acid for 20-30 minutes, adding potassium permanganate to solution, placing solution in water bath at 35-38 degrees C, mixing for 2-3 hours, adding deionized water to mixed solution, mixing at 90-95 degrees C for 15-20 minutes, adding hydrogen peroxide to solution, mixing at room temperature, filtering solution, washing with dilute hydrochloric acid, centrifuging solution with deionized water until the detergent is neutral, drying sample in vacuum oven at 50-60 degrees C to obtain graphene oxide, placing graphene oxide in 80 ml deionized water, performing ultrasonic treatment for 8-10 minutes, adding hydrazine hydrate, placing in reactor, soaking in deionized water for 24-30 hours, freeze-drying to obtain graphene foam, adding zirconium (IV) oxychloride octahydrate and graphene foam to deionized water, performing ultrasonic treatment for 10-15 minutes, adding 3 mol/L phosphoric acid, mixing for 2-3 hours, dissolving in reactor, reacting for 24-36 hours at 160-180 degrees C, filtering solution, washing for 3-5 times with anhydrous alcohol and ionic water, drying sample in oven at 60-65 degrees C to obtain alpha -ZrP-graphene foam, adding alpha -ZrP/graphene foam material in 80 ml deionized water, performing ultrasonic treatment for 30-40 minutes, adding ferric chloride hexahydrate to mixture, performing ultrasonic treatment for 5-8 minutes, adding pyrrole aqueous solution to solution, mixing at 45-50 degrees C for 70-90 minutes, washing product with deionized water for 3 times to obtain modified alpha -ZrP/graphene foam, placing modified alpha -ZrP/graphene foam in deionized water, performing ultrasonic treatment for 5-8 minutes to obtain suspension, polishing glassy carbon electrode with aluminum oxide powder, performing ultrasonic treatment in ethanol and ultrapure water for 3-5 minutes, drying with nitrogen, coating suspension droplet on the surface of working electrode with liquid transfer gun, drying at room temperature, modifying hemoglobin solution on the surface of working electrode and drying at 3-5 degrees degrees C. The mass ratio of potassium nitrate and graphite powder is 1:1.5-1:2. The mass ratio of graphite powder to potassium permanganate is 1:3.5-1:3.8. The volume ratio of deionized water and concentrated sulfuric acid is 1.2:1-1.5:1. The ratio of hydrazine hydrate to graphene oxide solution is 1:6-1:8. The mass ratio of graphene foams and zirconium (IV) oxychloride octahydrate is 1:0.48-1:0.63. The ratio of zirconium (IV) oxychloride octahydrate to phosphoric acid is 1:12-1:15. The mass ratio of alpha -ZrP/graphene foam amd pyrrole 1:1-1:1.3. The mass ratio of pyrrole to ferric chloride hexahydrate is 1:2.8-1:3.