▎ 摘　　要

NOVELTY - A graphene biosensor preparation method involves preparing ultrasonically dispersing graphite oxide in de-ionized water to get (0.0001-0.1 mg/ml) graphene oxide dispersion liquid, where ultrasonic frequency is 55kHz-60kHz, followed by adding supporting electrolyte (0-1 mol/l) and putting base electrode in the electrolyte solution. The obtained solution is subjected to electro-deposition at -0.9 to 1.2V for 10-50 seconds. The obtained electrode is washed by de-ionized water ad dried at room temperature, followed by subjecting graphene modified electrode to perform electric potential scanning. USE - Method for preparing a graphene biosensor. ADVANTAGE - The method enables preparing graphene biosensor with controlled density and better repeatability in an eco-friendly manner. DETAILED DESCRIPTION - A graphene biosensor preparation method involves preparing ultrasonically dispersing graphite oxide in de-ionized water to get (0.0001-0.1 mg/ml) graphene oxide dispersion liquid, where ultrasonic frequency is 55kHz-60kHz, followed by adding supporting electrolyte (0-1 mol/l) and putting base electrode in the electrolyte solution. The obtained solution is subjected to electro-deposition at -0.9 to 1.2V for 10-50 seconds. The obtained electrode is washed by de-ionized water ad dried at room temperature, followed by subjecting graphene modified electrode to perform electric potential scanning at 0.4V for 10-50 seconds in 0.002-0.2 mmol/l chloroauric acid solution. The obtained electrode is washed by de-ionized water and dried at room temperature. The obtained electrode is subjected to cyclic volt-ampere scanning in conductive macromolecule monomer (0.01-0.2 mol/l) and electrolyte (0.001-0.005 mol/l) at 0-4 degrees C for 2-10 times, where scanning rate is 10 to 400mV/second and volt-ampere scanning voltage range is 0-1.0V. The obtained electrode is washed by de-ionized water and dried at room temperature. The obtained electrolyte is placed in a 18-22mM ethylene dichloride/ N-hydroxysuccinimide solution for 4-6 hours and soaked in a (8-12 mg/ml) catalase at 4 degrees C for 22-26 hours, followed by washing with de-ionized water. The obtained graphene biosensor is subjected to analyze heat dissipation performance of the electrochemical biosensor in graphene testing device. DESCRIPTION OF DRAWING(S) - The drawing shows a flowchart illustrating a method for preparing graphene biosensor.'(Drawing includes non-English language text)'