• 专利标题:   Electrochemical immunosensor for detecting aminophenylarsinic acid comprises nanocomposite material, Staphylococcus protein A s fixed on the nano-composite material, and then the antiaminobenzene arsonic acid monoclonal antibody is added dropwise.
  • 专利号:   CN114113263-A
  • 发明人:   MA D, ZHU X, LIANG C, CHEN Y, ZHOU J, WANG A, YOU X, ZHANG G
  • 专利权人:   UNIV ZHENGZHOU
  • 国际专利分类:   G01N027/327
  • 专利详细信息:   CN114113263-A 01 Mar 2022 G01N-027/327 202281 Chinese
  • 申请详细信息:   CN114113263-A CN11476487 06 Dec 2021
  • 优先权号:   CN11476487

▎ 摘  要

NOVELTY - Electrochemical immunosensor for detecting aminophenylarsinic acid comprises nanocomposite material, Staphylococcus protein A s fixed on the nano-composite material, and then the antiaminobenzene arsonic acid monoclonal antibody is added dropwise. The outermost layer is bovine serum albumin, reduced graphene oxide (rGO)- polyethyleneimine (PEI)-silver-Nf composite solution includes reduced graphene oxide, polyethyleneimine and silver nanoparticles and perfluorosulfonic acid. USE - Used as electrochemical immunosensor for detecting aminophenylarsinic acid. ADVANTAGE - The immunosensor has excellent conductivity. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: 1 preparing the electrochemical immunosensor for detecting aminophenylarsinic acid, comprising (i) carrying out gold electrode pretreatment, (ii) preparing the rGO-PEI-Ag-Nf composite solution, dropping solution on the gold electrode pretreated in the step (i), and drying at room temperature, and testing the current of the electrode in phosphate-buffered saline solution, (iii) dropping 0.1-0.8 mg/ml Staphylococcus protein A on the surface of the electrode obtained in the step (ii), incubating at 30°C and 40°C for 50-70 minutes, rinsing the electrode surface, drying, and testing current changes in phosphate-buffered saline solution, (iv) dropping the anti-aminophenylarsinic acid monoclonal antibody onto the electrode surface obtained in the step (iii), incubating at 37°C for 30-50 minutes, rinsing the electrode surface, drying, and testing the change value of the current, (v) dropping 0.25 wt.% bovine serum albumin on the electrode surface obtained in the step (iv) for incubating, rinsing the electrode surface after incubation for 20-40 minutes, and drying to obtain an electrochemical immunosensor for detecting aminophenylarsinic acid; and 2 method for detecting aminophenylarsonic acid by electrochemical immune sensor for detecting aminophenylarsonic acid.