▎ 摘　　要

NOVELTY - Graphene nanopore sequencer comprises probe nano-motion platform, AFM probe, a nanopore chip, a hydrophobic liquid tank, and a three-dimensional nano-motion platform. On the needle nano-motion platform, the hydrophobic liquid tank is fixed on the three-dimensional nano-motion platform, and the nanopore chip is installed in the hydrophobic liquid tank. The tip of the AFM probe is provided with a DNA tetrahedron. The nano A solid nanopore is etched on the hole chip, and the solution in the hydrophobic liquid tank communicates through the nanopore, and a number of magnetic beads are provided in the solution below the nanopore. USE - As graphene nanopore sequencer. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for nanopore sequencing method based on the graphene nanopore sequencer, which involves clamping the processed and unmodified DNA tetrahedron AFM probe on the probe nano motion platform, scanning the experimental area to accurately locate the nanopore and fixing the position of the AFM probe, injecting modification solution to assemble and capture the DNA tetrahedron and the DNA in sequence with the tip of the AFM probe, using modification chain through on DNA tetrahedron under probe to determine whether the DNA tetrahedron is successfully modified by blocking the current, binding the DNA to be tested, injecting test liquid above and below the nanopore chip, arranging streptavidin magnetic beads under the nanopore chip, applying voltage on both sides of the nanopore to drive the DNA on the tip surface of the AFM probe through the nanopore and captured by the streptavidin on the surface of the magnetic bead under the action of electric field force, straightening the DNA, controlling the probe nano-motion platform, and vertically downwarding AFM probe to realize DNA sequencing process.