▎ 摘　　要

NOVELTY - Sensing of single molecule involves contacting a molecular sensor with one charge modulator, where contacting generates a change in current, measuring the change in current, and correlating the change in current with the presence or absence of one charge modulator and sensing the single molecule. USE - Method for sensing of single molecule using chemically-sensitive field effect transistor device. ADVANTAGE - The method is configured to detect 1-200 single molecules per second, provide improved retrieval of biomolecule-based information, allows to establish the identity of 100 bases, and improve nucleic acid sequencing through use of nanoelectric sensing devices. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: Chemically-sensitive field effect transistor device (200) for sensing single molecules, which comprises a solid support (204), where solid support comprises loci, where each loci comprises graphene layer (201), gate electrode (203a) and drain electrode (203b), where gate electrode and drain electrode are in electrical communication via the graphene layer, and one insulating layer (202a), where insulating layer is located between the gate electrode and the drain electrode, and loci have a pitch of 50-1000 nanometer; and Method for single molecule polynucleotide sequencing, which involves: (a) contacting polynucleotides with at least one primer and one polymerase to form ternary complexes, where ternary complex comprises a graphene binder; (b) detecting one or more bases of the polynucleotides in real time, where detection occurs when multiple ternary complexes are bound to the graphene layer of the device; and (c) removing the ternary complexes from the surface; and repeating steps to sequence the polynucleotides. DESCRIPTION OF DRAWING(S) - The drawing shows schematic view of a chemically-sensitive field effect transistor device for sensing single molecules. 200Chemically-sensitive field effect transistor device 201Graphene layer 203aGate electrode 203bDrain electrode 204Solid support