• 专利标题:   Preparing magnetic graphene molecular engram Raman enhancing substrate comprises e.g. ultrasonically dispersing iron(III) chloride hexahydrate, iron(II) chloride tetrahydrate in water, adding water and stirring under nitrogen atmosphere.
  • 专利号:   CN108318421-A
  • 发明人:   SHE Y, ZHAO F, HONG S, ZHANG C, WANG S, WANG J, ZHENG L, JIN M, JIN F, SHAO H
  • 专利权人:   INST QUALITY STANDARDS TESTING TECHNOL
  • 国际专利分类:   G01N021/01, G01N021/65
  • 专利详细信息:   CN108318421-A 24 Jul 2018 G01N-021/01 201865 Pages: 14 Chinese
  • 申请详细信息:   CN108318421-A CN11405366 22 Dec 2017
  • 优先权号:   CN11405366

▎ 摘  要

NOVELTY - Preparing magnetic graphene molecular engram Raman enhancing substrate comprises e.g. ultrasonically dispersing 2.3-2.4 pts. wt. iron(III) chloride hexahydrate, 0.8-0.9 pts. wt. iron(II) chloride tetrahydrate in 10 pts.vol water, adding 80 pts.vol water, stirring at 80 degrees C for 10 minutes under nitrogen atmosphere, adding 10 pts.vol 25% aqueous ammonia, stirring at 80 degrees C for 30 minutes, cooling and magnetically separating to obtain ferrous oxide magnetic nanoparticles, ultrasonically dispersing 0.2 pts. wt. ferrous oxide magnetic nanoparticles, 160 pts.vol ethanol and 80 pts.vol water, adding 5 pts.vol 25% aqueous ammonia, adding 0.7 pts.vol tetraethoxysilane, stirring, reacting at room temperature for 12 hours and magnetically separating to obtain ferrous oxide-silicon dioxide particles, ultrasonically dispersing 0.2 pts. wt. ferrous oxide-silicon dioxide particles in water, adding 5 pts.vol 25% ammonia, 60 pts. wt. water, 120 pts.vol 50% ethanol solution and ultrasonically dispersing. USE - The engram Raman enhancing substrate is useful in detecting Raman spectroscopy (claimed). DETAILED DESCRIPTION - Preparing magnetic graphene molecular engram Raman enhancing substrate comprises (i) ultrasonically dispersing 2.3-2.4 pts. wt. iron(III) chloride hexahydrate, 0.8-0.9 pts. wt. iron(II) chloride tetrahydrate in 10 pts.vol water, adding 80 pts.vol water, stirring at 80 degrees C for 10 minutes under nitrogen atmosphere, adding 10 pts.vol 25% aqueous ammonia, stirring at 80 degrees C for 30 minutes, cooling and magnetically separating to obtain ferrous oxide magnetic nanoparticles, (ii) ultrasonically dispersing 0.2 pts. wt. ferrous oxide magnetic nanoparticles, 160 pts.vol ethanol and 80 pts.vol water, adding 5 pts.vol 25% aqueous ammonia, adding 0.7 pts.vol tetraethoxysilane, stirring, reacting at room temperature for 12 hours and magnetically separating to obtain ferrous oxide-silicon dioxide particles, (iii) ultrasonically dispersing 0.2 pts. wt. ferrous oxide-silicon dioxide particles in water, adding 5 pts.vol 25% ammonia, 60 pts. wt. water, 120 pts.vol 50% ethanol solution, ultrasonically dispersing, adding 0.4 pts.vol 3-aminopropyltriethoxysilane, stirring, reacting at 40 degrees C for 24 hours and magnetically separating to obtain ferrous oxide-silicon-amino, (iv) ultrasonically dispersing 0.015 pts. wt. graphene oxide and 30 pts.vol water, add 0.01 pts. wt. ethylene dichloride and 0.008 pts. wt. N-hydroxysuccinimide, ultrasonically stirring for 30 minutes to obtain a suspension, adding 0.1-0.15 pts. wt. ferrous oxide-silicon dioxide-amino particles, ultrasonically dispersing, stirring at 80 degrees C for 1 hours and magnetically separating to obtain ferrous oxide-silicon dioxide-graphene oxide, (v) dissolving 0.0294 pts. wt. triazolone as a template molecule in 10 pts.vol acetonitrile, adding methacrylic acid, pre-polymerizing on a shaker for 20 minutes to obtain a prepolymerization reaction solution, where molar ratio between methacrylic acid and template molecule is 6:1; separating 0.05-0.1 pts. wt. ferrous oxide-silicon dioxide-graphene oxide in 10 pts. wt. acetonitrile to obtain a suspension, adding suspension into the prepolymerization solution, adding ethylene glycol dimethacrylate, 0.04 pts. wt. azobisisobutyronitrile, ultrasonicating for 5 minutes, passing nitrogen and oxygen for 5 minutes, sealing, placing in a water bath at 60 degrees C, shaking for 24 hours, reacting and then magnetically separating to obtain ferrous oxide-silicon dioxide-graphene oxide-molecularly imprinted polymer, where molar ratio between ethylene glycol dimethacrylate and template molecule is 10:1. INDEPENDENT CLAIMS are also included for: (1) magnetic graphene molecular engram Raman enhancing substrate is obtained by above method; and (2) use of graphene molecular engram Raman enhancing substrate in detecting Raman spectroscopy.