▎ 摘　　要

NOVELTY - Method for measuring estradiol using graphene multiple signal amplification surface plasmon resonance (SPR) sensing, involves preparing sample using magnetic graphene as the adsorbent, adsorbing and enriching estradiol in the sample, eluting, fixing the eluted estradiol to the volume to obtain sample, washing surface of the precious metal sheet of the SPR chip, connecting carboxylated graphene oxide on the surface of the precious metal sheet, using carboxylated graphene oxide fixed on the surface of the precious metal of the SPR chip to connect and fix E2-BSA, using SPR immunosensor to determine the concentration of estradiol in the test sample based on the indirect competition method, mixing the test sample containing E2 and the monoclonal antibody solution of E2 for injection, and jointly competing on the surface of the SPR chip for E2-BSA, where the signal generated by SPR is inversely proportional to the concentration of E2 in the sample. USE - The method is useful for measuring estradiol (claimed).