▎ 摘　　要

NOVELTY - Graphene-stripping hydrotalcite sheet composite immobilized protein modified electrode comprises base electrode layer and graphene-stripping hydrotalcite sheet composite fixed protein layer. The electrode is prepared by adding cobalt chloride hexahydrate (CoCl2.6H2O), aluminum chloride (AlCl3.6H2O) and urea in deionized water in round-bottomed flask with total metal ion concentration of 0.15 mol/L, refluxing, filtering, washing filter cake, drying, dispersing in sodium chloride (NaCl) and hydrochloric acid (HCl) mixed aqueous solution, stirring, suction filtering and drying. USE - Graphene-stripping hydrotalcite sheet composite immobilized protein modified electrode used for preparing electrochemical sensor in detecting trichloroacetic acid (all claimed). ADVANTAGE - The electrode has synergistic effect and low detection limit. It increases ELDH conductivity and inhibits ELDH aggregation and accumulation inhibits re-graphene sheets stacking, reducing contact resistance between sheets and improves electron transfer rate. DETAILED DESCRIPTION - Graphene-stripping hydrotalcite sheet composite immobilized protein modified electrode comprises base electrode layer and graphene-stripping hydrotalcite sheet composite fixed protein layer. The electrode is prepared by adding divalent metal salt cobalt chloride hexahydrate (CoCl2.6H2O), trivalent metal salt aluminum chloride hexahydrate (AlCl3.6H2O) and urea in certain molar ratio to deionized water in round-bottomed flask with total metal ion concentration of 0.15 mol/L, refluxing while stirring at 60-98 degrees C for 10-36 hours, filtering, washing filter cake with distilled water for 3-5 times, drying at room temperature for 48 hours to get carbonate intercalated layered double hydroxides (LDH), dispersing carbonate intercalated LDH in 1 mol/L sodium chloride (NaCl) and 3.3 mmol/L hydrochloric acid (HCl) mixed aqueous solution, stirring under nitrogen atmosphere for 12 hours, suction filtering, drying at room temperature for 12 hours to get chloride intercalated LDH, dispersing chloride intercalated LDH in 0.1 mol/L sodium nitrate (NaNO3), stirring under nitrogen atmosphere for 12 hours, suction filtering, drying at room temperature for 12 hours to get nitrate intercalated LDH, dispersing nitrate intercalated LDH in 1 mg/mL formamide, stirring under nitrogen atmosphere for 24 hours, centrifuging at 2000 revolutions/minute (rpm) for 10 minutes, discarding precipitate to remove non-peeled product to get stripped product exfoliated LDH (ELDH) colloid; using hummer method, ultrasonic dispersing graphene oxide (GO) in 1 mg/mL formamide for 1 hour, centrifuging at 3000 rpm for 10 minutes to non-peeling GO to get stripped GO dispersion liquid, mixing stripped GO dispersion liquid and ELDH colloid, stirring at room temperature for 12 hours under sealing, centrifuging at 5000 rpm for 10 minutes, redispersing resulting brown precipitate in deionized water, reacting with hydrazine hydrate at 80 degrees C for 2.5 hours while stirring and centrifuging at 4000 rpm for 10 minutes to get graphene ELDH (GRELDH) nano hybrid materials; adding graphite powder with ionic liquid BPPF6 in mass ratio of 2:1 in mortar, heating at 80 degrees C, mixing uniformly, adding 1 mL liquid paraffin as binder dropwise, grinding, filling in glass tube and inserting copper wire to get carbon ionic liquid electrode (CILE), where before using electrode polished surface first until smooth; and mixing GRELDH nano hybrid materials in deionized water to get 1 mg/mL slurry, coating CILE surface with 8 mu L slurry, drying naturally, coating with 8 mu L 10 mg/mL Hb solution, drying naturally, coating with 6 mu L 1 mg/mL chitosan (CTS) acetate solution and drying naturally.