▎ 摘　　要

NOVELTY - Method (M1) for constructing a graphene oxide-based nucleic acid aptamer sensor involves (a) modifying the carboxyl-X-rhodamine fluorescent group at 5' end of the nucleic acid aptamer to obtain a fluorescently labeled nucleic acid aptamer, and (b) conjugating and adsorbing graphene oxide and fluorescently labeled nucleic acid aptamer to perform fluorescence quenching to obtain a graphene oxide-based nucleic acid aptamer sensor. USE - The method (M1) is useful for constructing graphene oxide-based nucleic acid aptamer sensor, which is useful for detecting food safety, and detecting content of fumonisin B1 in a wheat flour sample (all claimed). ADVANTAGE - The method has high sensitivity and high specificity, quickly detects content of fumonisin B1 in wheat powder, has high accordance with the ELISA detection kit, and has important practical application value in the food safety. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a graphene oxide-based nucleic acid aptamer sensor being obtained by the construction method (M1); (2) a method (M2) for verifying graphene oxide-based nucleic acid aptamer sensor involves (i) reacting the graphene oxide-based nucleic acid aptamer sensor with the target fumonisin B1, and (ii) digesting the nucleic acid aptamer with endonuclease DNaseI, and reacting to release the target fumonisin B1 to realize the amplification of the target cyclic signal; and (3) a method (M3) for detecting fumonisin B1 using the graphene oxide-based nucleic acid aptamer sensor involves (a) configuring the fumonisin B1 standard solution of different concentration gradients, utilizing the graphene oxide-based nucleic acid aptamer sensor to analyze, detecting the fluorescence signal intensity and spectral curve, and fitting the standard curve, and (b) pretreating the actual sample, using the graphene oxide-based nucleic acid aptamer sensor for detection, and determining the content of fumonisin B1 based on linear model of the standard curve.