▎ 摘　　要

NOVELTY - Detecting pesticide acetamiprid by porous silicon micro-cavity device comprises (a) etching the Bragg reflectors with the same upper and lower periods of high and low refractive index alternately arranged and the porous silicon of a high-porosity cavity layer in the middle, (b) functionalizing the corroded porous silicon, (c) coupling the functionalized porous silicon with the nucleic acid aptamer of acetamiprid, (d) taking the quantum graphene, adding e.g. N-hydroxy succinimide (NHS) to activate the carboxy, adding the activated quantum dot into the complementary chain solution of acetamiprid aptamer modified by amino, (e) dropping the complementary strand marked by the quantum dot on the porous silicon micro-cavity coupled with the aptamer, (f) dropping the acetamiprid solution to the surface of the porous silicon micro-cavity coupled with the hybridization chain and (g) detecting the porous silicon micro-cavity by using reflectance method and realizing acetamiprid detection. USE - The method is useful for detecting the concentration of pesticide molecules. ADVANTAGE - The method: couples the nucleic acid aptamer to the porous silicon microcavity, hybridizing the complementary chain modified with graphene quantum dots with the aptamer, so the effective refractive index increases; separates the aptamer from the complementary chain of the coupled graphene quantum dots and combined with acetamiprid, when the acetamiprid sample was added, because of its stronger specificity to the aptamer, resulting in a decrease in the effective refractive index; increases the change of effective refractive index with the increase of the concentration of acetamiprid solution, increasing the change of central wavelength and obtains the spectral images before and after the reaction by the reflectance spectrometer, so as to obtain the moving change of the central wavelength and the measured pesticide acetamiprid concentration relationship. DETAILED DESCRIPTION - Detecting pesticide acetamiprid by porous silicon micro-cavity device comprises (a) etching porous silicon: using anodic electrochemical corrosion method, etching the Bragg reflectors with the same upper and lower periods of high and low refractive index alternately arranged and the porous silicon of a high-porosity cavity layer in the middle, (b) functionalization of porous silicon: functionalizing the corroded porous silicon, (c) fixing the aptamer on the porous silicon: coupling the functionalized porous silicon with the nucleic acid aptamer of acetamiprid, (d) activating and marking the quantum dot: taking the quantum graphene, respectively adding 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxy succinimide (NHS) to activate the carboxy, adding phosphate buffered saline (PBS) to dilute, adding the activated quantum dot into the complementary chain solution of acetamiprid aptamer modified by amino, making the quantum dot mark complementary strand, (e) hybridization of aptamer and complementary strand: dropping the complementary strand marked by the quantum dot on the porous silicon micro-cavity coupled with the aptamer, (f) coupling of acetamiprid and aptamer: dropping the acetamiprid solution to the surface of the porous silicon micro-cavity coupled with the hybridization chain and (g) acetamiprid detection: detecting the porous silicon micro-cavity by using reflectance method, obtaining the change relation of the variation and refractive index of the micro-cavity central wavelength and realizing acetamiprid detection.