▎ 摘　　要

NOVELTY - A hemoglobin-nano palladium-graphene composite material is prepared by grinding graphite powder, 1-hexyl pyridine hexafluorophosphate (HPPF6) and liquid paraffin, filling into glass pipe for compaction, embedding copper to obtain carbon ion liquid electrode (CILE), front polishing to mirror surface, preparing nano-palladium-graphene (Pd-GR) solution, ultrasonically dispersing hemoglobin (Hb), oscillating and uniformly mixing by drip-painting to modify carbon ion liquid electrode surface, and drying at room temperature. USE - Method for preparing hemoglobin-nano palladium-graphene composite material used for detecting electro-catalytic reduction of trichloroacetic acid and sodium nitrite (all claimed). ADVANTAGE - The method increases transfer rate of electrons between hemoglobin and substrate electrode. DETAILED DESCRIPTION - A hemoglobin-nano palladium-graphene composite material is prepared by grinding 1.6 g graphite powder, 0.8 g 1-hexyl pyridine hexafluorophosphate (HPPF6) and 500 mu L liquid paraffin into carbon paste, filling into glass pipe for compaction, embedding copper to obtain carbon ion liquid electrode (CILE), front polishing to mirror surface, preparing nano-palladium-graphene (Pd-GR) solution, ultrasonically dispersing hemoglobin (Hb) to obtain nano-Hb-Pd-GR mixed solution, oscillating and uniformly mixing by drip-painting to modify carbon ion liquid electrode surface, drying at room temperature, dropping Nafion (RTM: perfluoro-sulfonic acid polymer) coating, and drying to obtain electrochemical biosensor element to prepare Nafion (RTM: perfluoro-sulfonic acid polymer)/CILE, Nafion (RTM: perfluoro-sulfonic acid polymer)/Pd-GR/CILE and Nafion (RTM: perfluoro-sulfonic acid polymer)/Hb/CILE modified electrode.