• 专利标题:   Electrochemical device based on eliminating small molecule electrodes, has dehydrogenase small molecule enzyme membrane electrode including cellulose acetate membrane, polycarbonate microporous membrane and fixed rubber ring.
  • 专利号:   CN113758982-A, CN113758982-B
  • 发明人:   ZHOU X, SONG H, ZHU Z
  • 专利权人:   TIANJIN INST IND BIOTECHNOLOGY
  • 国际专利分类:   G01N027/327, G01N027/48
  • 专利详细信息:   CN113758982-A 07 Dec 2021 G01N-027/327 202233 Chinese
  • 申请详细信息:   CN113758982-A CN10566310 24 May 2021
  • 优先权号:   CN10566310

▎ 摘  要

NOVELTY - The device has a device shell including a bottom plate (1) provided with a hole that has the functions of both the liquid inlet a and the air extraction hole a. The middle-layer separator (8) separates the elimination small molecule reaction chamber (3) and the enzymatic reaction chamber (11). The top plate (12) is provided with a suction hole c and an installation hole for the enzyme membrane electrode. The enzymatic reaction chamber sequentially includes a dehydrogenase-small molecule enzyme membrane electrode arranged on the top plate as a working electrode, a reference electrode b (17), and a counter electrode b (18). The reference electrode b is a silver wire, and the counter electrode b is a platinum wire. The dehydrogenase small molecule enzyme membrane electrode comprises a base electrode, a cellulose acetate membrane, an immobilized enzyme layer, a polycarbonate microporous membrane and a fixed rubber ring connected in sequence. USE - Electrochemical device based on eliminating small molecule electrodes used for detection of target substances in fermentation broth (claimed) used in bio-fermentation industry and industries of food, feed, medicine, chemical industry, and energy. ADVANTAGE - The fermentation liquid to be tested enters the small molecule elimination reaction chamber and the enzymatic reaction chamber in turn, and the device is used to detect the fermentation liquid sample through the combination of a dehydrogenase small molecule enzyme membrane electrode and an electrode for eliminating small molecules by an electrochemical method. The interference of small molecules in the actual sample can be effectively eliminated, and the detection accuracy can be improved. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for an application of the electrochemical device based on eliminating small molecule electrodes in detecting target substances in fermentation broth. DESCRIPTION OF DRAWING(S) - The drawing shows schematic diagram of an electrochemical device. (Drawing includes non-English language text) Bottom plate (1) Filtering film a (2) Elimination small molecule reaction chamber (3) Waste liquid discharge outlet (5) Middle-layer separator (8) Enzymatic reaction chamber (11) Top plate (12) Pumping port pipeline (13) Valve (14) Cleaning liquid sample inlet b (15) Waste liquid discharge outlet b. silver wire (16) Reference electrode b (17) Counter electrode b (18)