• 专利标题:   Chemical sensor element useful in chemical sensor array for detecting target substances e.g. odorant substance in gas phase, comprises sensitive membrane, on which recombinant olfactory receptor is immobilized, provided on insulating interface, source electrode and drain electrode.
  • 专利号:   WO2023026843-A1
  • 发明人:   YOSUKE F, KENZO M, MASAFUMI Y, TAKASHI I, IKUMI T, TOMOYA Y
  • 专利权人:   UNIV TOKYO AGRIC TECHNOLOGY
  • 国际专利分类:   C07K017/00, C12N015/12, G01N027/00, G01N027/414
  • 专利详细信息:   WO2023026843-A1 02 Mar 2023 G01N-027/414 202329 Pages: 62 Japanese
  • 申请详细信息:   WO2023026843-A1 WOJP030344 08 Aug 2022
  • 优先权号:   JP136417

▎ 摘  要

NOVELTY - Chemical sensor element comprises a sensitive membrane, on which a recombinant olfactory receptor is immobilized, is provided on an insulating interface and source electrode and drain electrode provided in contact with the sensitive membrane. USE - The chemical sensor element is useful in chemical sensor array for detecting target substances in gas phase. The target substance is an odorant substance (claimed). ADVANTAGE - The chemical sensor element detects a target substance, such as an odorant substance, in a gas phase with highly sensitivity. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: a chemical sensor array for detecting target substances in gas phase comprising a chemical sensor element, and two or more types of immobilized recombinant olfactory receptors as an index, for each chemical sensor element mounted on the chemical sensor array, a wiring group connected to the source electrode, and a wiring group connected to the drain electrode so as to be electrically independent from each other and to enable electrical connection with the outside; chemical sensor system for detecting target substances in the gas phase comprising a scanning circuit electrically connected to the source electrode group of the chemical sensor array, and a detection circuit electrically connected to the drain electrode group, and unit provided above detection circuit for performing a computational process of receiving an electrical signal generated when the recombinant olfactory receptor immobilized on the chemical sensor element mounted on the chemical sensor array comes into contact with a substance in the gas phase, detecting substance from received electrical signal and displaying the substance derived by the computation process as a target substance; and a method for producing a chemical sensor element, involves providing a sensitive membrane to which a recombinant olfactory receptor is immobilized on an insulating interface, expressing gene encoding the olfactory receptor in a cell-free expression system to obtain a recombinant olfactory receptor, immobilizing the recombinant olfactory receptor on the sensitive membrane provided on the insulating interface and preparing a chemical sensor element provided with a sensitive membrane on which a recombinant olfactory receptor is immobilized.