▎ 摘　　要

NOVELTY - Angiotensin-converting enzyme ACE functionalized magnetic nano-microsphere comprises magnetic graphene oxide, tetraethyl orthosilicate, aminopropyltriethoxysilane to prepare angiotensin-converting enzyme ACE. USE - Used as angiotensin-converting enzyme ACE functionalized magnetic nano-microsphere.. ADVANTAGE - The method quickly and efficiently screens out 5 ACE inhibitory active ingredients from the jujube extracting solution, the synthesized ACE functionalized magnetic nano-sphere can be specifically screened, is featured with good precision and high efficiency. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: preparing angiotensin-converting enzyme ACE functionalized magnetic nanospheres (1) synthesis of magnetic graphene oxide by magnetic graphene oxide MGO nanoparticles were prepared by Fe3+ and Fe2+ chemical co-precipitation method, dispersing graphene oxide GO, Iron trichloride hexahydrate (FeCl 3.6H2O) and Iron(II) chloride tetrahydrate (FeCl2.4H2O) in distilled water, reaction conditions nitrogen protection to isolate the air and heating and stirring, adjusting the pH of the mixed solution to alkaline conditions and continue stirring, after the reaction is completed, filtering the suspension, separating the magnetic material with the help of an external magnetic field, wash with distilled water and ethanol, drying and grind to obtain a magnetic oxide Graphene MGO nanoparticles, (2) synthesis of magnetic graphene oxide wrapped in SiO2 by measuring tetraethyl orthosilicate, adding distilled water and stir ultrasonically, adding the obtained milky white solution and magnetic graphene oxide to absolute ethanol and mechanically stir, gradually drop adding glutaraldehyde solution to continue the reaction, separating with a magnet, and the synthesized GO doped Fe3O4 doped SiO2 (SMGO) nanoparticles, washing with 2% nitric acid, distilled water and absolute ethanol in sequence, and the product is obtained after drying, (3) synthesis of ACE-functionalized magnetic nanospheres by dispersing SMGO nanoparticles in ethanol-aqueous solution, adjusting the pH to acidic with 0.1 mol/l hydrochloric acid, slowly adding aminopropyltriethoxysilane (APTES) and stir to continue the reaction, separating the synthesized product by a magnet, washing with ethanol and drying, the dried solid is dispersed in a 5% glutaraldehyde solution, separating by a magnet to recover SMGO-NH 2 nanoparticles, washing with Tris-HCl buffer, and removing excess glutaraldehyde, mixing with ACE-containing buffer and shakening to synthesize ACE-functionalized magnetic microspheres (SMGO-ACE); Use of angiotensin-converting enzyme ACE functionalized magnetic nano-microspheres in capture active components in the jujube extract and quickly screen out potential ACE enzyme inhibitors comprising (1) magnetic nanospheres capture active ingredients comprising extracting jujube powder with ethanol and repeating 3 times, mixing extracts, filtering, freeze-drying, and dissolving in methanol, washing ligand bound by angiotensin-converting enzyme with Tris-HCl buffer to remove the non-specific binding ligand, and the extracted ligand is eluted by incubating with methanol, (2) UPLC-Q-Exactive orbitrap-MS/MS qualitative analysis of active ingredients and eluting, filtering through a 0.22 μm membrane and injecting into the UPLC-Q-Exactive orbitrap-MS/MS system to qualitatively analyze the active ingredients captured by magnetic nanospheres, and further validation of the specificity of the synthesized magnetic microspheres for angling ligands.