▎ 摘　　要

NOVELTY - Preparation of disposable mercury ion sensor involves adding 5.0 mu L of 0.5 mg/mL graphene oxide solution in the hole on the surface of working electrode and drying, using silver/silver chloride electrode as reference electrode and using saturated calomel electrode as a counter electrode, and graphene oxide/indium tin oxide electrode as working electrode and electrochemically depositing gold-platinum alloy in a solution by cyclic voltammetry, dripping 10.0 mu L bovine serum albumin on the surface of obtained electrode, incubating, washing the obtained electrode with phosphate buffered saline, adding 5.0 mu L of different concentrations of mercury(II) ion standard solution and 5.0 mu L anti-mercury (II) ion and polyclonal antibody, incubating and washing with phosphate buffered saline times, adding 10.0 mu L horseradish peroxidase-labeled antibody to 1.0 mL of antibody, shaking and adding 10.0 mu L on the surface of obtained electrode, incubating, and washing with phosphate buffered saline. USE - Preparation of disposable sensor used for detecting mercury ions. ADVANTAGE - The disposable mercury ion sensor obtained from the method is capable of generating a current signal, and achieving improved linear range of signal value and mercury ion concentration and lower detection limit. DETAILED DESCRIPTION - Preparation of disposable mercury ion sensor involves (i) cutting indium tin oxide glass electrodes into 12x 20 mm thin slices and cleaning and ultrasonically processing in a water bath using acetone, ethanol, and double distilled water for 10 minutes, and placing in an oven for drying, (ii) using a hole punch to punch a 4 mm diameter hole on the transparent tape, and pasting on the baked tin oxide glass to fix the electrode reaction area to obtain an indium tin oxide working electrode, (iii) adding 5.0 mu L of 0.5 mg/mL graphene oxide solution in the hole on the surface of the working electrode and drying under infrared lamp to form a graphene oxide/indium tin oxide electrode, (iv) using silver/silver chloride electrode as reference electrode and using saturated calomel electrode as a counter electrode, and graphene oxide/indium tin oxide electrode as working electrode and electrochemically depositing gold-platinum alloy in a solution comprising 1.0 mg/mL chloroauric acid and 1.0 mg/mL chloroplatinic acid by cyclic voltammetry to obtain graphene oxide-gold-platinum nanocomposite to form graphene oxide-gold-platinum/indium tin oxide electrode, (v) dissolving 0.05 mmol methylmercury chloride in 200.0 mu L methanol solution comprising 1.0 mol/L sodium hydroxide, adding 5.0 mL 6-mercaptonicotinic acid at a concentration of 0.01 mmol/L and mixing with 200.0 mu L ovalbumin at a concentration of 1.0 mg/mL to obtain mercury-ovalbumin-based material as a coating antigen, and washing the graphene oxide-gold-platinum/indium tin oxide electrode with 20.0 mu L phosphate buffered saline, washing three times, dripping 10.0 mu L mercury-ovalbumin-based material at a concentration of 20.0 g/mL to the obtained electrode, and incubating overnight at 4 degrees C to form mercury-ovalbumin-based graphene oxide-gold-platinum/indium tin oxide electrode, (vi) dripping 10.0 mu L bovine serum albumin at a concentration of 20.0 mu g/mL on the surface of mercury-ovalbumin-based graphene oxide-gold-platinum/indium tin oxide electrode, incubating for 30 minutes at 4 degrees C to block non-specific active sites on the surface of mercury-ovalbumin-based graphene oxide-gold-platinum/indium tin oxide electrode, (vii) washing the obtained sealed mercury-ovalbumin-based graphene oxide-gold-platinum/indium tin oxide electrode with phosphate buffered saline for three times, adding 5.0 mu L of different concentrations of mercury(II) ion standard solution and 5.0 mu L anti-mercury (II) ion and polyclonal antibody at a concentration of 20.0 mu g/mL and incubating for 30 minutes at 4 degrees C and washing with phosphate buffered saline for three times, and (viii) adding 10.0 mu L horseradish peroxidase-labeled antibody to 1.0 mL of antibody, shaking and adding 10.0 mu L on the surface of obtained electrode, incubating for 30 minutes at 4 degrees C, and washing with phosphate buffered saline for three times. An INDEPENDENT CLAIM is included for usage method of disposable mercury ion sensor, which involves using an electrochemical workstation to perform the measurement using a three-electrode system, preparing 5.0 mL phosphate-sodium chloride buffer comprising 1% hydrogen peroxide and 5% 3,3',5,5'-tetramethylbenzidine, and adjusting the pH to 7.0 using 1.0 mol/L sodium hydroxide as a buffer solution, using differential pulse voltammetry to test in the buffer, drawing working curve through the relationship between the current signal and the mercury ion standard solution concentration, and testing the sample solution.