▎ 摘　　要

NOVELTY - Constructing an immobilized multi-enzyme system based on the interaction between DNA, graphene oxide and metal organic framework materials, comprises weighing zirconium oxychloride octahydrate and meso-tetra(4-carboxyphenyl) porphine, dissolving ultrasonically in N,N-dimethylformamide (DMF), and adding dichloroacetic acid and stirring. The solution is transferred to the reactor and reacted at 130 degrees C for 18h, and after the reaction, the nanocrystals sre collected by centrifugation, the added mass ratio of zirconium oxychloride octahydrate, meso-tetra(4-carboxyphenyl) porphine, DMF and dichloroacetic acid is 76:13:3080 0:780. USE - Method for constructing an immobilized multi-enzyme system based on the interaction between DNA, graphene oxide and metal organic framework materials. ADVANTAGE - The method enables to construct an immobilized multi-enzyme system based on the interaction between DNA, graphene oxide and metal organic framework materials, which. DETAILED DESCRIPTION - Constructing an immobilized multi-enzyme system based on the interaction between DNA, graphene oxide and metal organic framework materials, comprises weighing zirconium oxychloride octahydrate and meso-tetra(4-carboxyphenyl) porphine, dissolving ultrasonically in N,N-dimethylformamide (DMF), and adding dichloroacetic acid and stirring. The solution is transferred to the reactor and reacted at 130 degrees C for 18h, after the reaction, the nanocrystals sre collected by centrifugation, the added mass ratio of zirconium oxychloride octahydrate, meso-tetra(4-carboxyphenyl) porphine, DMF and dichloroacetic acid is 76:13:3080 0:780. The nanocrystals are subjected to three solvent exchanges with DMF, and washed twice with ethanol and buffer A alternately, and after centrifugation, buffer A is added to disperse, and the PCN-222 stock solution is obtained and stored at 4 degrees C for later use. The 4 (N maleimidomethyl)cyclohexane 1 carboxylic acid sulfosuccinimide ester sodium salt (suflo SMCC) is dissolved ultrasonically with bufferA, and added to the above GOX solution, and the mixed solution is placed in a shaker and incubated at 37 degrees C for 2 hours. The mixture is filtered and washed with a 10K ultrafiltration tube to remove unreacted suflo-SM CC, and the mass ratio of suflo-SM CC to G Ox is 1:2. Single-stranded DNA (ssDNA) is vortexed with buffer A until it is completely dissolved. Tris (2-carboxyethyl) phosphine (TCEP) is weighed and added to buffer A for ultrasonic dissolution. The above two solutions are mixed and placed in a shaker at 37 degrees C, incubated for 2hours, and after the reaction, the mixture is filtered and washed with a 3K ultrafiltration tube to remove the TCEP that has not participated in the reaction. The filtered GOX solution and ssDNA solution is mixed in a shaker and incubated at 29 degrees C for 12h, and after the reaction is completed, filtered and washed with a 10K ultrafiltration tube to remove unreacted ssDNA, and the obtained GOX-ssDNA complex is stored at 4 degrees C for later use. The synthesis method of horseradish peroxidase (HRP)-ssDNA complex is the same as GOX-ssDNA complex, except that GOX is replaced with HRP. Graphene oxide (GO) is uniformly dispersed by ultrasonic in buffer A and stored at 4 degrees C for later use. The PCN-222, GOX-ssDNA complex, HRP-ssDNA complex, and GO solution is placed in a shaker, incubated at 29 degrees C for 3hours, rinsed with bufferA, and obtained an immobilized multi-enzyme system based on JanusDNA.