▎ 摘　　要

NOVELTY - Anisotropic film structure color hydrogel has a reduced graphene oxide sheet structure as a substrate, and an upper surface of the substrate has adjacent optical sensing regions and cell growth regions. The optical sensing region includes a first hydrogel and an etched structure of colloidal particles distributed in the first hydrogel, and the cell growth region includes a second hydrogel doped with reduced graphene oxide and distributed in a second hydrogel. USE - Anisotropic film structure color hydrogel for detecting cardiomyocytes, and screening and evaluating cardiac drugs (all claimed). ADVANTAGE - The anisotropic film structure color hydrogel is prepared under low cost, does not need large detecting instrument, ensures visual observation of the adjustability, has high efficiency of sensor attribute, good and stable optical property, is flexible, does not relyon large detecting instrument, and realize the myocardial cell pulsation frequency and contractile force. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) preparing anisotropically-colored hydrogel film, which involves using colloidal particle self-assembly method to prepare colloidal crystal array template and reduced graphene oxide sheet structure respectively;placing colloidal crystal array template and reduced graphene oxide sheet structure parallel to each other with a gap in the middle, and the area of the reduced graphene oxide sheet structure is larger than the colloidal crystal array template;filling the first hydrogel prepolymer solution into the above colloidal crystal array template and polymerizing;placing a patterned mask on the non-colloidal crystal array template area directly above the reduced graphene oxide sheet structure, where a mixed solution of a second hydrogel precursor and reduced graphene oxide is injected in the middle, and the two gels form a mixed template;removing colloidal crystal array template from the mixed template to obtain a graphene hybrid structure color hydrogel having an inverse opal structure, that is, an optical sensing region, and a pattern corresponding to the topological structure, that is, a cell growth region; and (2) application of the anisotropically-colored hydrogel film in detecting the frequency and contractile force of myocardial cells, which involves culturing myocardial cells by using the anisotropic structure color hydrogel film, where the contraction and relaxation of cardiomyocytes on the hydrogel film cause periodic changes in the characteristic reflection peaks with an inverse opal structure, and collect the data of the characteristic reflection peaks; analyzing the periodic change data of the characteristic reflection peak collected to obtain the contraction force and frequency of the cardiac muscle cells.