▎ 摘　　要

NOVELTY - Method for preparing a reversible calcium(2+) ions recognition ligand (METH) involves (a) mixing amine substance (I) and diglycolic anhydride in dichloromethane, stirring and reacting to obtain 2-(2-(dicyclohexylamino)-2-oxoethoxy)acetic acid, and (b) mixing the 2-(2-(dicyclohexylamino)-2-oxoethoxy)acetic acid, amine substance (II), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and hydroxybenzotriazole in dichloromethane, stirring and reacting to obtain the product. USE - The method is useful for preparing a reversible calcium(2+) ions recognition ligand, which is useful in preparation of a calcium(2+) ions sensor and/or a reversible calcium(2+) ions selective electrode (METH-E) (all claimed). ADVANTAGE - The ligand (METH) has high selectivity and high reversibility, carries alkynyl linking group, which has the characteristics of being able to be connected to the gold surface, realizes long-term reversible real-time detection of calcium(2+) ions concentration. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a method for preparing a reversible calcium (2+) ions selective electrode (METH-E) involving (a1) using a 3-electrode system containing a reference electrode, immersing the carbon fiber electrode in a chloroauric acid solution at room temperature, using an electrochemical workstation to connect the electrode and setting up constant potential electrodeposition to obtain a gold-plated carbon fiber electrode, (b1) immersing the gold-plated carbon fiber electrode in the graphene oxide suspension, using an electrochemical workstation to connect the electrode, setting up constant potential electrodeposition to obtain a gold-plated carbon fiber electrode coated with graphene oxide, and (c1) immersing the prepared gold-plated carbon fiber electrode coated with graphene oxide in an ethanol solution containing METH, soaking in an oxygen-free environment, and drying in air to obtain the product; and (2) use of reversible calcium(2+) ion selective electrode in measuring calcium(2+) ions and/or in monitoring and drug mechanism research.