• 专利标题:   New polypeptide modified graphene quantum dot GSG useful in preparation of lysine fluorescence detection reagent.
  • 专利号:   CN108609617-A
  • 发明人:   CHENG R, LI M, ZHEN Z, ZHENG D, PEI S, DAI L
  • 专利权人:   UNIV WENZHOU MEDICAL
  • 国际专利分类:   C01B032/194, C09K011/65, C09K011/02, C07K005/037, G01N021/64
  • 专利详细信息:   CN108609617-A 02 Oct 2018 C01B-032/194 201901 Pages: 7 Chinese
  • 申请详细信息:   CN108609617-A CN10518729 25 May 2018
  • 优先权号:   CN10518729

▎ 摘  要

NOVELTY - A polypeptide modified graphene quantum dot GSG (I), is new. USE - The polypeptide modified graphene quantum dot GSG is useful in preparation of lysine fluorescence detection reagent (claimed). ADVANTAGE - The polypeptide modified graphene quantum dot GSG detects lysine with high selectivity. DETAILED DESCRIPTION - A polypeptide modified graphene quantum dot GSG of formula (I), is new. INDEPENDENT CLAIMS are included for the following: (1) a method for producing the polypeptide-modified graphene quantum dot GSG, involves taking 35 ml 0.05-5.5 mg/ml aqueous solution of graphene quantum dot in 100 ml beaker, dropwise adding 0.2-0.35 ml mixed solvent of N-hydroxysuccinimide and dicyclohexylcarbodiimide, allowing to stand for 10-15 minutes, weighing 0.1-0.2 g glutathione dissolved in 5 ml deionized water, dropwise adding to activated graphene quantum dots, ultrasonically dispersing in a 37 degrees C water bath for 10 minutes, heating at 55 degrees C for 3 hours, stirring in water bath at room temperature for 24 hours, dialyzing using dialysis bag having molecular weight cutoff of 1000 using 1000 ml deionized water for three days, and changing water every 3 hours; and (2) use of the polypeptide-modified graphene quantum dot in preparation of lysine fluorescence detection reagent.