• 专利标题:   Glutathione detection kit for determining glutathione, comprises tetramethylbenzidine hydrochloride-hydrogen peroxide solution, glutathione solution as standard stock solution and graphene oxide platinum nanocomposite solution.
  • 专利号:   CN107144662-A
  • 发明人:   CHEN W, DENG H, LIN X, PENG H
  • 专利权人:   UNIV FUJIAN MEDICAL
  • 国际专利分类:   B82Y015/00, B82Y030/00, C01B032/198, G01N031/10
  • 专利详细信息:   CN107144662-A 08 Sep 2017 G01N-031/10 201776 Pages: 9 Chinese
  • 申请详细信息:   CN107144662-A CN10246612 16 Apr 2017
  • 优先权号:   CN10246612

▎ 摘  要

NOVELTY - Glutathione detection kit comprises graphene oxide platinum nanocomposite solution as liquid (a), 3,3',5,5'-tetramethylbenzidine hydrochloride-hydrogen peroxide solution as liquid (b), and glutathione solution as standard stock solution. The graphene oxide platinum nanocomposite is prepared by adding 9.225 mL 2 mg/mL graphene oxide solution to 0.575 mL 10 mg/mL aqueous solution of chloroplatinic acid, mixing vigorously for 15 minutes, dripping 0.2 mL 5 mg/ml sodium borohydride solution, magnetically stirring and freeze-drying. USE - Glutathione detection kit is used for determining glutathione in blood samples (claimed). ADVANTAGE - The glutathione detection kit efficiently detects glutathione in the detection limit of 4 nmol/L. DETAILED DESCRIPTION - Glutathione detection kit comprises graphene oxide platinum nanocomposite solution as liquid (a), 3,3',5,5'-tetramethylbenzidine hydrochloride-hydrogen peroxide solution as liquid (b), and glutathione solution as standard stock solution. The graphene oxide platinum nanocomposite is prepared by adding 9.225 mL 2 mg/mL graphene oxide solution to 0.575 mL 10 mg/mL aqueous solution of chloroplatinic acid, mixing vigorously for 15 minutes, dripping 0.2 mL 5 mg/ml sodium borohydride solution, magnetically stirring, and freeze-drying. An INDEPENDENT CLAIM is included for determination of glutathione using glutathione detection kit in the absorbance measuring wavelength of 652 nm.