▎ 摘　　要

NOVELTY - Synthesizing fluorescent signal amplifier comprises grafting an amino-containing spacer on the active site of the graphene oxide surface, modifying the boric acid on the spacer arm of the graphene oxide, loading fluorescent substance on the boric acid modified graphene oxide, and wrapping the hydrophilic polymer substance, the method specifically comprises (1) grafting graphene oxide raw material with amino spacers on the surface, where the raw material includes graphene oxide, 2-morpholineethanesulfonic acid, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide, sodium dihydrogen phosphate, disodium hydrogen phosphate, double distilled water, (2) modifying boric acid graphene oxide, (3) loading boron affinity graphene oxide raw material with fluorescent substance, (4) wrapping boron affinity graphene oxide raw material with hydrophilic polymer material and loading with fluorescent material. USE - The method is useful for synthesizing fluorescent signal amplifier, which is useful for determining glycoprotein content. ADVANTAGE - The method: has high detection sensitivity to the glycoprotein, wide detection range, and has short time consumption compared with enzyme-linked immunosorbent assay technology; is simple, economical, and has good stability. DETAILED DESCRIPTION - Synthesizing fluorescent signal amplifier comprises grafting an amino-containing spacer on the active site of the graphene oxide surface, modifying the boric acid on the spacer arm of the graphene oxide, loading fluorescent substance on the boric acid modified graphene oxide, and wrapping the hydrophilic polymer substance, the method specifically comprises (1) grafting graphene oxide raw material with amino spacers on the surface, where the raw material includes 0.04-0.07 wt.% graphene oxide, 0.18-0.25 wt.% 2-morpholineethanesulfonic acid, 0.05-0.07 wt.% 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, 0.12-0.15 wt.% N-hydroxysuccinimide , 0.10-0.25 wt.% amino spacer material, 0.30-0.45 wt.% sodium dihydrogen phosphate, 0.70-0.85 wt.% disodium hydrogen phosphate , 89.50-98.50 wt.% double distilled water, (2) modifying boric acid graphene oxide, and raw material includes 0.045-0.070 wt.% surface grafted graphene oxide containing amino spacers, 0.85-1 wt.% sodium cyanoborohydride, 0.10-0.15 wt.% 2,4-difluoro-3-formylphenylboronic acid or 2,6-difluoro-4-formylphenylboronic acid, and 90.50-98.83 wt.% ethanol, (3) loading boron affinity graphene oxide raw material with fluorescent substance includes 0.050-0.065 wt.% modified graphene oxide, 0.11-0.15 wt.% fluorescent substance, 2.5-3 wt.% dimethyl sulfoxide, 0.30-0.40 wt.% Sodium dihydrogen phosphate, 0.65-0.75 wt.% disodium hydrogen phosphate, and 89.5-96.5 wt.% double distilled water, (4) wrapping boron affinity graphene oxide raw material with hydrophilic polymer material and loading with fluorescent material includes 0.050-0.065 wt.% boron affinity graphene oxide loaded with fluorescent material, 0.095-0.150 wt.% hydrophilic polymer material, 0.30-0.40 wt.% sodium dihydrogen phosphate, 0.65-0.75 wt.% disodium hydrogen phosphate, and 89.5-98.5 wt.% double distilled water. An INDEPENDENT CLAIM is also included for a method for using fluorescent signal amplifier comprising (1) adding the synthesized fluorescent signal amplifier to the solution containing the immobilized glycoprotein, binding the boronic acid monomer grafted by the fluorescent signal amplifier to the cis-dihydroxyl group of the glycoprotein under physiological pH conditions, (2) washing, adding a release agent to release fluorescein from the surface of the graphene oxide into the solution, and (3) detecting with a fluorescence spectrophotometer at a specific excitation/emission wavelength to achieve quantitative detection of glycoprotein content