• 专利标题:   Preparing porous graphene, comprises e.g. inoculating spore suspension of white rot fungi to seed culture medium containing bacterial cellulose film, culturing, immobilizing fungal membrane in liquid nutrient limitation medium, and heating.
  • 专利号:   CN106350544-A
  • 发明人:   ZHU C, YANG C, YANG M, CAO Y
  • 专利权人:   HEFEI GUOXUAN HIGH TECH POWER SOURCE CO
  • 国际专利分类:   C12P003/00, C12R001/645
  • 专利详细信息:   CN106350544-A 25 Jan 2017 C12P-003/00 201733 Pages: 8 Chinese
  • 申请详细信息:   CN106350544-A CN10720170 24 Aug 2016
  • 优先权号:   CN10720170

▎ 摘  要

NOVELTY - Preparing porous graphene, comprises (i) inoculating the spore suspension of white rot fungi into a seed culture medium containing bacterial cellulose film, and culturing; (ii) immobilizing immobilized white rot fungal membrane by placing into a liquid nutrient limitation medium; and (iii) removing the degraded graphene film from the thermostatic shaking incubator, peeling out mycelium adsorbed outside graphene oxide using tweezers, placing peeled hyphae into dilute hydrochloric acid, heating to dissolve, centrifuging, washing to obtain pure porous graphene suspension; and drying. USE - The method is useful for preparing porous graphene (claimed). ADVANTAGE - The method is environmental-friendly (claimed), carried out under mild reaction conditions, and economical. DETAILED DESCRIPTION - Preparing porous graphene, comprises (i) inoculating the spore suspension of white rot fungi into a seed culture medium containing bacterial cellulose film, and culturing in a constant temperature shaking incubator, to obtain an immobilized white rot fungal membrane; (ii) immobilizing immobilized white rot fungal membrane by placing into a liquid nutrient limitation medium, replacing in the constant temperature oscillation incubator for 1-3 days, reacting for 5-24 hours by adding graphene oxide suspension, allowing the adsorption of graphene on the surface of immobilized white rot fungus membrane, carrying out biological oxidation and degradation, to obtain degraded graphene film; and (iii) removing the degraded graphene film from the thermostatic shaking incubator, peeling out mycelium adsorbed outside graphene oxide using tweezers, placing peeled hyphae into dilute hydrochloric acid, heating to dissolve, centrifuging, washing to obtain pure porous graphene suspension; and drying to obtain the final product.