• 专利标题:   Microfluidic system e.g. microfluidic cartridge for impedance-based detection of biological entity in sample, has common electrode to electrically communicate output signal to impedance analyzer, where output signal correlates to impedance variation caused by displacement of biological entity.
  • 专利号:   WO2023086733-A2
  • 发明人:   JAVANMARD M, SUI J, AZIZPOUR S, LIAN Z
  • 专利权人:   UNIV RUTGERS STATE NEW JERSEY
  • 国际专利分类:   G01N000/00
  • 专利详细信息:   WO2023086733-A2 19 May 2023 G01N-000/00 202345 Pages: 62 English
  • 申请详细信息:   WO2023086733-A2 WOUS078708 26 Oct 2022
  • 优先权号:   US272414P

▎ 摘  要

NOVELTY - The microfluidic system has excitation electrodes (131-133) that are respectively coupled to each of the microfluidic flow channels (121-123) and electrically connected to a signal generator. The excitation electrodes are configured to receive and electrically communicate an excitation signal applied by the signal generator to each of the microfluidic flow channels, where the excitation signal generates an electric field between each of the excitation electrodes and the common electrode (140). The common electrode is coupled to all of the microfluidic flow channels and electrically connected to an impedance analyzer. The common electrode is configured to electrically communicate an output signal to the impedance analyzer. The output signal is correlated to an impedance variation caused by displacement of the biological entity within each of the microfluidic flow channels. USE - The microfluidic system e.g. microfluidic cartridge or microfluidic chip is useful for performing impedance-based detection of biological entity in sample, identifying or counting a biological entity in sample, identifying or diagnosing a disease or disorder in a subject, and monitoring progression of disease or disorder. The biological entity is (i) a bacterium, a virus, a protein, a microparticle, a nanoparticle, a nucleic acid, a biomarker or a bead with biological material, or (ii) red blood cell, white blood cell, platelet, hematocrit, hemoglobin, neutrophil, lymphocyte or microbial. The disease or disorder is a bacterial or viral infection, preferably influenza or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The sample comprises a bodily fluid, preferably blood (all claimed). ADVANTAGE - The impedance-based detection of biological entity in the sample can be performed easily and efficiently. The simultaneous measurements of a sample in two or more microfluidic flow channels are enabled to minimize faulty results. The need of lysing samples and measuring multiple times that is more time-consuming, is eliminated and could introduce some variations across samples and devices. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: a kit of microdluidic system; a method for identifying or counting a biological entity in a sample; a method of diagnosing a disease or disorder in a subject; and a method of monitoring progression of a disease or disorder in a subject. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic diagram of the microfluidic cartridge. 100Microfluidic cartridge 121-123Microfluidic channels 131-133Excitation electrodes 140Common electrode 180Lock-in amplifier