▎ 摘　　要

NOVELTY - Detecting microRNA (miRNA) based on graphene oxide/nucleic acid dye detection platform and nucleic acid constant temperature amplification technique, comprises designing DNA hairpin probe sequence according to the to-be detected target miRNA, carrying out terminal modification of DNA hairpin probes 5' end and/or 3' end, carrying out strand displacement constant temperature amplification reaction, adding nucleic acid dye, graphene oxide solution and assay buffer, and placing the product in a fluorescence analyzer, and measuring the intensity of the fluorescence signal. USE - The method is useful for detecting microRNA (miRNA) based on graphene oxide/nucleic acid dye detection platform and nucleic acid constant temperature amplification technique. ADVANTAGE - The method utilizes non-modified probe with high sensitivity and specificity for simple and rapid detection of target molecules. DETAILED DESCRIPTION - Detecting microRNA (miRNA) based on graphene oxide/nucleic acid dye detection platform and nucleic acid constant temperature amplification technique, comprises (i) designing DNA probe card by designing DNA hairpin probe sequence according to the to-be detected target miRNA, and carrying out terminal modification of DNA hairpin probes 5' end and/or 3' end, (ii) using strand displacement constant temperature amplification reaction primer in the designed DNA hairpin probe sequence, (iii) using the reaction system comprising analyte of the target miRNA, designed DNA hairpin probes and primers, the nucleic acid amplification enzyme with strand displacement nature, 4 types of deoxyribonucleotides and the reaction buffer to carry out strand displacement constant temperature amplification reaction, (iv) adding nucleic acid dye, graphene oxide solution and detection buffer solution into the constant temperature amplification product, and incubating, and (v) placing the product obtained in step (iv) in a fluorescence analyzer, measuring the intensity of the fluorescence signal according to the value and the standard curve, which is capable of detecting the amount of a target miRNA in the system.