▎ 摘　　要

NOVELTY - Reactive oxygen species (ROS)-sensitive graphene-based nanomaterial is prepared by introducing graphene-based nanomaterials onto Pluronic (RTM: Poloxamer)-modified polyamidoamine (PAMAM) polymer by ROS-sensitive double selenium bond linker. USE - The ROS-sensitive graphene-based nanomaterial is useful as drug carrier (claimed). ADVANTAGE - The ROS-sensitive graphene-based nanomaterial is prepared simply, has good dispersibility and low cell toxicity, and enables to rapidly release the drug until therapeutic concentration is attained. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) method for preparing the ROS-sensitive graphene-based nanomaterial, involves (a) adding activated graphene oxide into L-selenocystine solution under protection of inert gas, stirring at room temperature to react for 12-36 hours, collecting and washing to obtain L-cystine modified-graphene oxide, and (b) dispersing the L-cystine modified-graphene oxide in a buffer, adding Pluronic (RTM: Poloxamer)-modified PAMAM polymer, agitating uniformly and then adding N-hydroxysuccinimide/1-ethyl-3-3(-dimethylaminopropyl)carbodiimide hydrochloride solution under inert gas protection, reacting at room temperature for 12-36 hours, collecting and washing to obtain product; and (2) use of the ROS-sensitive graphene-based nanomaterial, as a drug carrier, by mixing the ROS-sensitive graphene-based nanomaterial with drug solution, stirring at room temperature for 12-48 hours, and performing ultrafiltration and centrifugal purification to obtain a drug-loaded complex.