▎ 摘　　要

NOVELTY - Sensor assembly (100) for detecting the presence of at least one amplification product of an isothermal amplification process, comprises: an amplification product receiving region; and a first sensor (120) responsive to the detection of an indicator of the presence of the amplification product to provide a first signal, where the first sensor comprises a sensing surface (120a) arranged to contact a sample in the amplification product receiving region, the sensing surface comprises a first layer (125) comprising a one-dimensional or two-dimensional material, and the first sensor is either a pH sensor adapted to detect a change in pH indicative of the presence of the amplification product, or a sensor adapted to detect the presence of the amplification product directly. The one-dimensional or two-dimensional material comprises graphene, hexagonal boron-nitride and/or carbon nanotubes, preferably single-walled carbon nanotubes. USE - The sensor assembly is useful: for detecting the presence of at least one amplification product of an isothermal amplification process; in a system for isothermal amplification of a sample; for determining the presence of an analyte in a sample; in a pH sensor (all claimed); and for detecting particular nucleic acid sequences in a sample e.g. in sample containing a virus. ADVANTAGE - The sensor assembly: provides improved isothermal amplification assays, which are quicker, cheaper and more suited to point-of-care and home testing environments; utilizes transducers (e.g. electrochemical sensors or chemiresistors) as the first sensor that enables quick yet precise detection of the amplification product(s) or an indicator of the presence of the amplification product(s), which provides a faster indication of whether the target of interest (e.g. a particular nucleic acid sequence) is present; can dramatically reduce measurement time, and even (combined with the accuracy and sensitivity of the sensors) enables detection to take place prior to the full completion of the isothermal amplification process; provides excellent performance (e.g. sensitivity and precision) under the conditions used in isothermal applications and for the detection of protons (e.g. in pH measurement); improves the accuracy of the assay and reduces the likelihood of false positives; and provides rapid and real-time results, which can provide the dual confirmation significantly faster (e.g. less than 1 minute) than existing single-detector systems. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: a system for isothermal amplification of a sample; determining the presence of an analyte in a sample; and a pH sensor. DESCRIPTION OF DRAWING(S) - The figure shows a schematic cross-sectional view of the sensor assembly. 100Sensor assembly 110Substrate 120First sensor 120aSensing surface 125First layer