• 专利标题:   Nucleic acid extraction reagent comprises graphene oxide, surfactant, and nuclease-free water.
  • 专利号:   CN112195175-A
  • 发明人:   LI Z, WENG G, WANG W, ZHU C, SONG L, HUANG Y
  • 专利权人:   ZHEJIANG ZHIYANG BIOLOGICAL TECHNOLOGY
  • 国际专利分类:   C12N015/10
  • 专利详细信息:   CN112195175-A 08 Jan 2021 C12N-015/10 202112 Pages: 6 Chinese
  • 申请详细信息:   CN112195175-A CN10607845 08 Jul 2019
  • 优先权号:   CN10607845

▎ 摘  要

NOVELTY - Nucleic acid extraction reagent comprises graphene oxide, surfactant, and nuclease-free water (balance amount). USE - Used as nucleic acid extraction reagent containing graphene oxide. ADVANTAGE - The reagent: cracks cell in biological sample in short time; is capable of absorbing protein adsorbed on the nucleic acid released by the specific adsorption cell after decomposing, making the nucleic acid fully free, which is convenient for grabbing ion column or magnetic bead so as to obtain pure nucleic acid. The method: is simple, ensures high yield, and suitable for molecular biological gene operation or clinical gene diagnosis field in various types of biological sample nucleic acid extraction and purification. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for use method of the nucleic acid extraction reagent, comprising cell lysis, nucleic acid adsorption, sample washing, and nucleic acid elution.