• 专利标题:   Identifying biomolecule by separating reservoir into two parts by graphene membrane, forming nanopore, coating graphene oxide layer in nanopore with organic layer to interact with biomolecule, and monitoring time trace of ionic current.
  • 专利号:   US2013256154-A1
  • 发明人:   PENG H
  • 专利权人:   INT BUSINESS MACHINES CORP
  • 国际专利分类:   B82Y030/00, B82Y040/00, B82Y005/00, G01N027/40, G01N033/50
  • 专利详细信息:   US2013256154-A1 03 Oct 2013 G01N-027/40 201368 Pages: 17 English
  • 申请详细信息:   US2013256154-A1 US464165 04 May 2012
  • 优先权号:   US435773, US464165

▎ 摘  要

NOVELTY - Method for identifying biomolecule involves configuring a reservoir separated into two parts by a graphene membrane, forming nanopore through the graphene membrane, coating the graphene oxide layer at the inner surface in the nanopore with an organic layer configured to interact with the biomolecules in a different way in order to differentiate the biomolecules, and monitoring time trace of ionic current to identify the biomolecule based on a respective interaction of the biomolecules with the organic layer. USE - The method is useful for identifying biomolecule (claimed) and DNA sequencing. ADVANTAGE - The method enables to identify biomolecule rapidly. DETAILED DESCRIPTION - Method for identifying biomolecule involves configuring a reservoir separated into two parts by a graphene membrane, forming nanopore through the graphene membrane, where the nanopore connects the two parts of the reservoir, the graphene membrane being distinct from and physically separated from electrodes transferring current, the nanopore and the two parts of the reservoir are filled with ionic buffer, and the graphene membrane comprises a graphene oxide layer at an inner surface in the nanopore, coating the graphene oxide layer at the inner surface in the nanopore with an organic layer configured to interact with the biomolecules in a different way in order to differentiate the biomolecules, where the organic layer enhances resolution and motion control of the biomolecule, and monitoring time trace of ionic current to identify the biomolecule based on a respective interaction of the biomolecules with the organic layer. An INDEPENDENT CLAIM is included for method for differentiating bases, which involves configuring a reservoir separated into two parts by membrane, forming nanopore through the membrane, where the nanopore connects the two parts of the reservoir, the nanopore and the two parts of the reservoir are filled with ionic buffer, and the membrane comprises a graphene layer or a graphene oxide layer, oxidizing the nanopore to graphene oxide at an inner surface, coating the graphene oxide in the nanopore with an organic layer configured to interact with bases of a molecule in a different way in order to differentiate the bases of the molecule, where the organic layer enhances resolution and motion control of the molecule in the nanopore, and monitoring a time trace of ionic current to identify the bases of the molecule based on a respective interaction of the bases with the organic layer.