▎ 摘　　要

NOVELTY - Composition comprises polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO). USE - The composition is useful in a PCR kit (claimed) for amplifying DNA, preferably genes having various sizes. ADVANTAGE - The composition: improves the speed and accuracy of PCR by increasing the efficiency and specificity of PCR by inhibiting primer dimerization and nonspecific binding of amplified amplicons; and promotes the denaturation of double-stranded DNA in a sample to shorten the PCR time. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a PCR kit, which comprises the composition; and (2) a PCR method, which involves preparing a PCR sample by mixing a DNA template, deoxynucleotide triphosphates (dNTPs), a DNA polymerase, and forward and reverse primers for amplifying a target sequence with the composition, and performing PCR using the prepared PCR sample.