▎ 摘　　要

NOVELTY - Separating beta-glucosidase fusion protein by foam comprises preparing crude enzyme solution containing fusion protein GLEGB by constructing expression fusion protein GLEGB plasmid, transferring expression in expression bacteria, crushing thalli, centrifuging and taking supernatant, the fusion protein GLEGB is the GLEGB of GIu-linker-ELP-GB fusion protein formed by connecting the elastic protein ELP, the graphene-binding peptide GB with the connecting peptide linker to the glucosaccharase Glu, (2) measuring the foam performance of crude enzyme solution using the foam analyzer to analyze the foam height and the change of the foam shape along with the time of the crude enzyme liquid sample with different concentrations, (3) initially separating and purifying the fusion protein GLEGB in the crude enzyme solution by using the foam separation device, (4) further separating and purifying the fusion protein GLEGB by combining with inverse transition cycling (ITC). USE - The method is useful for separating beta-glucosidase fusion protein by foam. ADVANTAGE - The method has high separation and purification efficiency, utilizes simple device, low investment, low energy consumption and no pollution, less enzyme activity loss and has great realistic significance. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for beta-glucosidase fusion protein prepared by above method.