• 专利标题:   Preparing three-dimensional printed hydrogel urethral stent useful in field of tissue engineering, e.g. bone and skin engineering, comprises e.g. placing frozen sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold into a vacuum freeze-drying machine, and crosslinking.
  • 专利号:   CN114681675-A
  • 发明人:   FU Q, CHEN J, YANG X, CHENG J, WANG L, LI W, ZHANG K
  • 专利权人:   ZHANG K
  • 国际专利分类:   A61L027/44, A61L027/52, A61L027/54, A61L027/56, A61L027/58, C08J003/075, C08L005/04, C08L089/00
  • 专利详细信息:   CN114681675-A 01 Jul 2022 A61L-027/44 202271 Chinese
  • 申请详细信息:   CN114681675-A CN10366052 08 Apr 2022
  • 优先权号:   CN10366052

▎ 摘  要

NOVELTY - Preparing three-dimensional printed hydrogel urethral stent comprises (1) preparing sodium alginate/gelatin/reduced graphene oxide composite hydrogel solution, (2) preparing sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold by 3D printing, (3) placing printed sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold in a low temperature environment of 85℃ to -80℃, and freezing for 2-2.5 hours, (4) placing frozen sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold into a vacuum freeze-drying machine for freeze-drying for 12-13 hours, (5) crosslinking freeze-dried sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold with 5% calcium chloride, crosslinking for 30-35 minutes, and using sterilized water for cleaning after crosslinking, and (6) lyophilizing washed sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold for second time, and storing in vacuum container. USE - The method is useful for preparing three-dimensional printed hydrogel urethral stent, which is useful in field of tissue engineering, e.g. bone and skin engineering. ADVANTAGE - The three-dimensional printed hydrogel urethral stent: has improved aperture size and tensile property.