▎ 摘 要
NOVELTY - Preparing three-dimensional printed hydrogel urethral stent comprises (1) preparing sodium alginate/gelatin/reduced graphene oxide composite hydrogel solution, (2) preparing sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold by 3D printing, (3) placing printed sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold in a low temperature environment of 85℃ to -80℃, and freezing for 2-2.5 hours, (4) placing frozen sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold into a vacuum freeze-drying machine for freeze-drying for 12-13 hours, (5) crosslinking freeze-dried sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold with 5% calcium chloride, crosslinking for 30-35 minutes, and using sterilized water for cleaning after crosslinking, and (6) lyophilizing washed sodium alginate/gelatin/reduced graphene oxide hydrogel scaffold for second time, and storing in vacuum container. USE - The method is useful for preparing three-dimensional printed hydrogel urethral stent, which is useful in field of tissue engineering, e.g. bone and skin engineering. ADVANTAGE - The three-dimensional printed hydrogel urethral stent: has improved aperture size and tensile property.