▎ 摘　　要

NOVELTY - A three-dimensional hydrogel-graphene-based biosensor comprises substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are sequentially stacked. The three-dimensional hydrogel material layer is composed of a three-dimensional network structure. The hydrogel material is formed by the polymerization reaction of raw materials including acrylamide monomer and modified probe molecules. The modified probe molecules are probe molecules modified with acrylamide groups. USE - Three-dimensional hydrogel-graphene-based biosensor. ADVANTAGE - The biosensor has good stability and high sensitivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for preparation of the biosensor comprising comprising preparing electrode layer on one side of the substrate to obtain a substrate-electrode layer device; disposing a graphene film on the surface of the electrode layer in the substrate-electrode layer device to obtain a substrate-electrode layer-graphene film device; and polymerizing reaction solution on the surface of the graphene film in the substrate-electrode layer-graphene film device to form a three-dimensional hydrogel material layer, where reaction solution contains acrylamide monomer, modified probe molecule, initiator, sodium nitrate and solvent.