▎ 摘　　要

NOVELTY - Preparing doxorubicin nanoparticles using hyaluronic acid-modified graphene oxide as a carrier, based on mg, g and mL involves cooling the ice-water bath container at 0-5 degrees C, and adding 40-50 mL of concentrated sulfuric acid. The 1-4g flake graphite powder and 0.4-2g sodium nitrate is added for ultrasonic treatment, stir the reaction, keep the reaction temperature below 10 degrees C, then weigh 4-8g potassium permanganate, add it in batches for 25-30 minutes, and react for 1.5-2.5 hours. The temperature is raised to 35-40 degrees C, and the reaction is performed for 25-30 minutes, measure 140-240mL of deionized water, cool to 0-5 degrees C in an ice-water bath. The temperature is adjust to 90-95 degrees C, and continue to stir the reaction for 25-30 minutes. The 100-140mL of water is add to stop the reaction, then add 40-60mL of hydrogen peroxide (H2O2) solution with a volume concentration of 25-35%, and continue to stir the reaction for 15-20 minutes. USE - Method for preparing doxorubicin nanoparticles using hyaluronic acid-modified graphene oxide as a carrier. ADVANTAGE - The method enables to prepare doxorubicin nanoparticles using hyaluronic acid-modified graphene oxide as a carrier do not affect the scientificity of the experimental results, has simple preparation processes, convenient operations, and do not require harsh reaction conditions and special reaction devices. DETAILED DESCRIPTION - Preparing doxorubicin nanoparticles using hyaluronic acid-modified graphene oxide as a carrier, based on mg, g and mL involves cooling the ice-water bath container at 0-5 degrees C, and adding 40-50 mL of concentrated sulfuric acid. The 1-4g flake graphite powder and 0.4-2g sodium nitrate ias added for ultrasonic treatment, stir the reaction, keep the reaction temperature below 10 degrees C, then weigh 4-8g potassium permanganate, add it in batches for 25-30 minutes, and react for 1.5-2.5 hours. The temperature is raised to 35-40 degrees C, and the reaction is performed for 25-30 minutes, measure 140-240mL of deionized water, cool to 0-5 degrees C in an ice-water bath. The temperature is adjust to 90-95 degrees C, and continue to stir the reaction for 25-30 minutes. The 100-140mL of water is add to stop the reaction, then add 40-60mL of hydrogen peroxide (H2O2) solution with a volume concentration of 25-35%, and continue to stir the reaction for 15-20 minutes. The 60-100mL 8-12% hydrogen chloride (HCl) solution by volume is added and let stand for 4-5 days. The supernatant of the solution is remove, then centrifuge the lower layer of viscous solution until the viscous solution is neutral, freeze-dry the viscous solution to obtain graphene oxide tablets. The graphene oxide sheet grounding into graphene oxide powder and obtain graphene oxide (GO) powder. The 150-200mg hyaluronic acid (HA) weigh in a container, dissolve it with 10-15mL formamide, seal the container, and oil bath at 45-55 degrees C until dissolved. The HA solution cool to room temperature, weigh 500-540mg 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), 300-320mg N-Hydroxysuccinimide (NHS), respectively, vortex with 8-12mL, then 3-7mL of formamide was vortex dissolved and added to the HA solution, and stirred at room temperature for activate carboxylic acids (COOH) 25-35 minutes to obtain an activated HA solution; The 10-15mL formamide solution in which 1-1.5mL ethylenediamine is dissolved in a container, drop the activated HA solution into the obtained ethylenediamine solution using a constant pressure dropping funnel under ice bath conditions, and add dropwise under bath conditions for 50-70 minutes. The reaction system is raised to room temperature and reacted for 2-4 hours. The acetone with a pre-cooled mass of 3-5 times added in the reaction solution in an ice bath, cool, crystallize, leave to stand, precipitate after the reaction is completed, 0.22 micrometer organic membrane suction filtration, wash the precipitate with acetone, remove the precipitate, and re- dissolve, dialysis in water, change water every 6-8 hours, freeze-dry after 48-60 hours to obtain the intermediate product HA-azanide (NH2). The 45-60mg of graphene oxide powder is weigh and put it in a container, add 30-40mL formamide, then transfer to another container after 25-35 minutes in an ice bath, rinse the previous container with 20-30mL formamide and rinse the solution, and transfer into the latter container. The 170-175mg of EDC, 100-105mg of NHS weigh and dissolve each with 5-10mL formamide, then add it to the latter container, and stir and activate at room temperature for 10-20 minutes to obtain an activated GO solution. The 90-135mg of HA-NH2 dissolved weigh in 10-15mL of formamide, add 180-270 microliter of triethylamine, after the activation is completed, add it dropwise to the activated GO solution, and react at room temperature for 20-30 hours. The 3-5 times the pre-cooled acetone added in an ice bath, cool, crystallize, stand, and precipitate, 0.22 micrometer organic membrane suction filtration, wash the precipitate with acetone, remove the precipitate, reconstitute with water, dialyze in water, replace water every 4-6 hours, and dialyze for 48-60 hours to obtain HA-GO solution. The 0.8-1 mL of doxorubicin taken with a concentration of 0.8-1.2 mg / mL into HA-GO solution, stir in the dark for 24-30 hours, centrifuge, collect the microparticles, freeze-dry the material in the lower layer, and save to obtain the target product.