▎ 摘　　要

NOVELTY - Method for preparing nanocomplex graphene hydrogel system involves reducing graphene oxide to graphene using hydrated hydrazine, loading hydrophobic drug paclitaxel (PTX), selecting thermosensitive N-Isopropylacrylamide (NIPAM) monomer, preparing single network hydrogel GSL, selecting acrylic acid monomer, synthesizing pH or temperature responsive graphite hydrogel GDL, incubating breast cancer MCF-7 cells with e.g. nanocomplex graphene hydrogel dual-drug system EPI-PTX-GDL, measuring cytotoxicity, and observing morphology of living cells. USE - The method is useful for preparing nanocomplex graphene hydrogel system (claimed), which is used for treating breast cancer. DETAILED DESCRIPTION - Method for preparing nanocomplex graphene hydrogel system involves modifying amphiphilic polymer PF127, reducing graphene oxide to graphene using hydrated hydrazine, loading hydrophobic drug paclitaxel (PTX) to obtain hydrophobic drug PTX-graphene, selecting thermosensitive N-Isopropylacrylamide (NIPAM) monomer as first network, and preparing single network hydrogel GSL by free radical polymerization, using PTX-graphene as an additive, selecting acrylic acid monomer with pH responsiveness as second network, synthesizing pH or temperature responsive graphite hydrogel GDL through ultraviolet irradiation, loading nanocomplex graphene hydrogel with hydrophilic drug epirubicin (EPI) into nanocomplex graphene hydrogel dual drug delivery system EPI-PTX-GDL, incubating breast cancer MCF-7 cells with dual-network hydrogel system EPI-GDL, PTX-GN, and nanocomplex graphene hydrogel dual-drug system EPI-PTX-GDL, measuring cytotoxicity by MTT assay, and observing morphology of living cells using fluorescence microscope.