▎ 摘　　要

NOVELTY - A modified electrode producing method involves pouring graphene oxide nano material solution on each glassy carbon electrode. The electrode is dried, washed using phosphate buffered saline, poured with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide and poured with PAC-1 antibody on each electrode, followed by storing electrode in humid chamber, rinsing with phosphate buffered saline, blocking using 2% bovine serum albumin solution inside humid chamber, rinsing bovine serum albumin blocked electrodes with milli-Q water and drying at room temperature in desiccators. USE - Method for producing modified electrode for use as biosensor that is utilized for electrochemical detection of activated platelets and platelet-derived microparticles. ADVANTAGE - The method enables producing modified electrode in an easy and cost effective manner with high sensitivity. DETAILED DESCRIPTION - A modified electrode producing method involves pouring graphene oxide nano material solution on each glassy carbon electrode after polishing electrode with aluminum slurry to cover the whole surface of electrode. The electrode is dried, washed with phosphate buffered saline, poured with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide to activate the carboxyl groups on graphene oxide, subjected for washing excess N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide by using phosphate buffered saline and poured with PAC-1 antibody on each electrode, followed by storing the electrode in humid chamber for 1 hour, rinsing the electrode with phosphate buffered saline, blocking using 2% bovine serum albumin solution inside humid chamber, rinsing bovine serum albumin blocked electrodes with milli-Q water and drying at room temperature in desiccators.